Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2365429..2366047 Replicon chromosome
Accession NZ_CP065615
Organism Escherichia coli strain FDAARGOS_943

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6H00_RS12245 Protein ID WP_001291435.1
Coordinates 2365829..2366047 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6H00_RS12240 Protein ID WP_000344800.1
Coordinates 2365429..2365803 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H00_RS12230 2360518..2361711 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I6H00_RS12235 2361734..2364883 + 3150 WP_197940073.1 multidrug efflux RND transporter permease subunit AcrB -
I6H00_RS12240 2365429..2365803 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6H00_RS12245 2365829..2366047 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6H00_RS12250 2366219..2366770 + 552 WP_000102564.1 maltose O-acetyltransferase -
I6H00_RS12255 2366886..2367356 + 471 WP_000136192.1 YlaC family protein -
I6H00_RS12260 2367520..2369070 + 1551 WP_038340860.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6H00_RS12265 2369112..2369465 - 354 WP_000878140.1 DUF1428 domain-containing protein -
I6H00_RS12275 2369844..2370155 + 312 WP_000409911.1 MGMT family protein -
I6H00_RS12280 2370186..2370758 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T183038 WP_001291435.1 NZ_CP065615:2365829-2366047 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T183038 NZ_CP086626:3922938-3923156 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT183038 WP_000344800.1 NZ_CP065615:2365429-2365803 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT183038 NZ_CP086626:3922538-3922912 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References