Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 7062..7819 Replicon plasmid unnamed
Accession NZ_CP065614
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name tacT Uniprot ID Q31SN7
Locus tag I6H01_RS24800 Protein ID WP_000501969.1
Coordinates 7334..7819 (+) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID Q31SN8
Locus tag I6H01_RS24795 Protein ID WP_011114751.1
Coordinates 7062..7346 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS24770 2332..2499 + 168 Protein_2 IS66 family insertion sequence element accessory protein TnpB -
I6H01_RS24775 2645..3664 + 1020 WP_038341671.1 IS110-like element ISEc11 family transposase -
I6H01_RS24780 3939..4121 + 183 WP_115209934.1 IS66 family insertion sequence element accessory protein TnpB -
I6H01_RS24785 4141..5742 + 1602 WP_004967149.1 IS66-like element ISSfl3 family transposase -
I6H01_RS24790 5996..6160 - 165 WP_001346193.1 hypothetical protein -
I6H01_RS24795 7062..7346 + 285 WP_011114751.1 DUF1778 domain-containing protein Antitoxin
I6H01_RS24800 7334..7819 + 486 WP_000501969.1 GNAT family N-acetyltransferase Toxin
I6H01_RS24805 7992..8324 - 333 Protein_9 IS4/IS5 family transposase -
I6H01_RS24810 8387..9543 + 1157 WP_086020934.1 IS3-like element IS600 family transposase -
I6H01_RS24815 9582..9722 - 141 Protein_11 IS3 family transposase -
I6H01_RS24820 9792..10979 - 1188 WP_181876988.1 hypothetical protein -
I6H01_RS24825 11049..12257 - 1209 WP_115209929.1 IS91 family transposase -
I6H01_RS24830 12238..12510 - 273 WP_000019158.1 hypothetical protein -
I6H01_RS24835 12677..12796 - 120 Protein_15 IS66 family insertion sequence hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB / ospE2 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / nleE / icsP/sopA / ospB / ospC4 / ospC4 / ospC2 / ospC2 / ospG / ipaH9.8 / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ 1..261275 261275
- inside IScluster/Tn - ospD3/senA / ospC1 1661..21144 19483


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(30-151)

Antitoxin

Vibrio phage ICP1, Orf50

(12-88)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17712.54 Da        Isoelectric Point: 10.1083

>T183025 WP_000501969.1 NZ_CP065614:7334-7819 [Escherichia coli]
MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATRTFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDVSFRGKGLGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
Q

Download         Length: 486 bp

>T183025 NZ_CP086626:c675551-675087 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10871.52 Da        Isoelectric Point: 4.6812

>AT183025 WP_011114751.1 NZ_CP065614:7062-7346 [Escherichia coli]
MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMACKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDP
AIEKLLARKPQWDV

Download         Length: 285 bp

>AT183025 NZ_CP086626:c675886-675551 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q31SN7

Antitoxin

Source ID Structure
AlphaFold DB A0A4V1CUJ1

References