Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4017548..4018166 Replicon chromosome
Accession NZ_CP065613
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6H01_RS19430 Protein ID WP_001291435.1
Coordinates 4017548..4017766 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6H01_RS19435 Protein ID WP_000344800.1
Coordinates 4017792..4018166 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS19395 4012837..4013409 + 573 WP_000779831.1 YbaY family lipoprotein -
I6H01_RS19400 4013440..4013751 - 312 WP_000409911.1 MGMT family protein -
I6H01_RS19410 4014130..4014483 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6H01_RS19415 4014525..4016075 - 1551 WP_038340860.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6H01_RS19420 4016239..4016709 - 471 WP_000136192.1 YlaC family protein -
I6H01_RS19425 4016825..4017376 - 552 WP_000102564.1 maltose O-acetyltransferase -
I6H01_RS19430 4017548..4017766 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6H01_RS19435 4017792..4018166 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6H01_RS19440 4018712..4021861 - 3150 WP_038340837.1 multidrug efflux RND transporter permease subunit -
I6H01_RS19445 4021884..4023077 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T183024 WP_001291435.1 NZ_CP065613:c4017766-4017548 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T183024 NZ_CP086626:263303-263410 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT183024 WP_000344800.1 NZ_CP065613:c4018166-4017792 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT183024 NZ_CP086626:c263254-263191 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References