183023

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 3981948..3982785 Replicon chromosome
Accession NZ_CP065613
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name itaT Uniprot ID A0A377CL67
Locus tag I6H01_RS19250 Protein ID WP_038341077.1
Coordinates 3981948..3982490 (-) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID A0A2X7FLN1
Locus tag I6H01_RS19255 Protein ID WP_038341078.1
Coordinates 3982474..3982785 (-) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS19230 3977488..3978399 - 912 WP_038341074.1 2-dehydropantoate 2-reductase -
I6H01_RS19235 3978567..3979058 + 492 WP_001138904.1 nucleotide binding protein YajQ -
I6H01_RS19240 3979186..3980550 - 1365 WP_038341075.1 MFS transporter -
I6H01_RS19245 3980957..3981892 + 936 WP_038341076.1 sel1 repeat family protein -
I6H01_RS19250 3981948..3982490 - 543 WP_038341077.1 GNAT family N-acetyltransferase Toxin
I6H01_RS19255 3982474..3982785 - 312 WP_038341078.1 DUF1778 domain-containing protein Antitoxin
I6H01_RS19260 3982970..3983860 - 891 WP_000971336.1 heme o synthase -
I6H01_RS19265 3983872..3984201 - 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
I6H01_RS19270 3984201..3984815 - 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
I6H01_RS19275 3984805..3986796 - 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
I6H01_RS19280 3986818..3987765 - 948 WP_038341083.1 cytochrome o ubiquinol oxidase subunit II -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19819.05 Da        Isoelectric Point: 8.3395

>T183023 WP_038341077.1 NZ_CP065613:c3982490-3981948 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGIARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T183023 NZ_CP086626:262820-262927 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11398.06 Da        Isoelectric Point: 10.4825

>AT183023 WP_038341078.1 NZ_CP065613:c3982785-3982474 [Escherichia coli]
MPGKTATLHDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT183023 NZ_CP086626:c262771-262708 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CL67

Antitoxin

Source ID Structure
AlphaFold DB A0A2X7FLN1

References