Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3136789..3137286 Replicon chromosome
Accession NZ_CP065613
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name ghoT Uniprot ID A0A377CNH6
Locus tag I6H01_RS15065 Protein ID WP_038341507.1
Coordinates 3137113..3137286 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag I6H01_RS15060 Protein ID WP_000398619.1
Coordinates 3136789..3137085 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS15040 3133520..3134239 - 720 WP_000611288.1 two-component system response regulator DcuR -
I6H01_RS15045 3134236..3135867 - 1632 WP_001216486.1 sensor histidine kinase -
I6H01_RS15050 3136048..3136278 + 231 WP_000371698.1 (4Fe-4S)-binding protein -
I6H01_RS15055 3136290..3136562 + 273 WP_000405647.1 N-acetyltransferase -
I6H01_RS15060 3136789..3137085 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
I6H01_RS15065 3137113..3137286 + 174 WP_038341507.1 type V toxin-antitoxin system toxin GhoT Toxin
I6H01_RS15070 3137405..3138922 - 1518 WP_001295089.1 lysine--tRNA ligase -
I6H01_RS15075 3139159..3140616 - 1458 WP_021557456.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6615.16 Da        Isoelectric Point: 10.2288

>T183019 WP_038341507.1 NZ_CP065613:3137113-3137286 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFFLF

Download         Length: 174 bp

>T183019 NZ_CP086625:c5051748-5051437 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTCACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT183019 WP_000398619.1 NZ_CP065613:3136789-3137085 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT183019 NZ_CP086625:c5051436-5051146 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377CNH6

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References