Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2365533..2366164 Replicon chromosome
Accession NZ_CP065613
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name hicA Uniprot ID A0A7U9LJQ2
Locus tag I6H01_RS11590 Protein ID WP_001259387.1
Coordinates 2365889..2366164 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID Q31VE4
Locus tag I6H01_RS11585 Protein ID WP_000593554.1
Coordinates 2365533..2365892 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS11555 2360645..2361118 + 474 WP_038339111.1 PTS sugar transporter subunit IIA -
I6H01_RS11560 2361115..2361396 + 282 WP_000084022.1 PTS sugar transporter subunit IIB -
I6H01_RS11565 2361473..2362831 + 1359 WP_001308116.1 PTS galactitol transporter subunit IIC -
I6H01_RS11570 2362824..2364332 + 1509 WP_001298718.1 carbohydrate kinase -
I6H01_RS11575 2364322..2364591 + 270 WP_000933060.1 HPr family phosphocarrier protein -
I6H01_RS11580 2364623..2365483 + 861 WP_001131788.1 class II aldolase -
I6H01_RS11585 2365533..2365892 - 360 WP_000593554.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6H01_RS11590 2365889..2366164 - 276 WP_001259387.1 type II toxin-antitoxin system HicA family toxin Toxin
I6H01_RS11595 2366237..2367361 - 1125 WP_001314210.1 ABC transporter permease -
I6H01_RS11600 2367361..2370096 - 2736 WP_038339112.1 ribosome-associated ATPase/putative transporter RbbA -
I6H01_RS11605 2370093..2371160 - 1068 WP_038339113.1 HlyD family secretion protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10214.91 Da        Isoelectric Point: 10.7076

>T183018 WP_001259387.1 NZ_CP065613:c2366164-2365889 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESQVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T183018 NZ_CP086625:c4755626-4755453 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13359.25 Da        Isoelectric Point: 4.8838

>AT183018 WP_000593554.1 NZ_CP065613:c2365892-2365533 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT183018 NZ_CP086625:c4755950-4755654 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LJQ2

Antitoxin

Source ID Structure
AlphaFold DB A0A6H2GM94

References