Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1712524..1713178 Replicon chromosome
Accession NZ_CP065613
Organism Escherichia coli strain FDAARGOS_944

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6H01_RS08345 Protein ID WP_000244777.1
Coordinates 1712524..1712931 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A377CRZ7
Locus tag I6H01_RS08350 Protein ID WP_038340210.1
Coordinates 1712912..1713178 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H01_RS08325 1708481..1710214 - 1734 WP_038340206.1 single-stranded-DNA-specific exonuclease RecJ -
I6H01_RS08330 1710220..1710930 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6H01_RS08335 1710955..1711851 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
I6H01_RS08340 1711963..1712484 + 522 WP_001055874.1 flavodoxin FldB -
I6H01_RS08345 1712524..1712931 - 408 WP_000244777.1 toxin CptA Toxin
I6H01_RS08350 1712912..1713178 - 267 WP_038340210.1 FAD assembly factor SdhE Antitoxin
I6H01_RS08355 1713421..1714401 + 981 WP_038340211.1 tRNA-modifying protein YgfZ -
I6H01_RS08360 1714478..1715137 - 660 WP_000250274.1 hemolysin III family protein -
I6H01_RS08365 1715301..1715612 - 312 WP_038340212.1 N(4)-acetylcytidine aminohydrolase -
I6H01_RS08370 1715657..1717090 + 1434 WP_038340246.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T183016 WP_000244777.1 NZ_CP065613:c1712931-1712524 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T183016 NZ_CP086625:c4509545-4509258 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10577.15 Da        Isoelectric Point: 5.1599

>AT183016 WP_038340210.1 NZ_CP065613:c1713178-1712912 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPTDAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT183016 NZ_CP086625:c4509271-4508969 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
AlphaFold DB A0A377CRZ7

References