Detailed information of TA system TA183012

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 721404..722239
• Replicon: chromosome
• Accession: NZ_CP065613
• Organism: Escherichia coli strain FDAARGOS_944

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A377EB25
• Locus tag: I6H01_RS03725
• Protein ID: WP_038341345.1
• Coordinates: 721862..722239 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: I6H01_RS03720
• Protein ID: WP_001295723.1
• Coordinates: 721404..721772 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H01_RS03690	717749..718702	+	954	Protein_721	autotransporter adhesin Ag43	-
I6H01_RS03695	718773..718877	+	105	WP_122999678.1	DUF905 family protein	-
I6H01_RS03700	719086..719904	+	819	WP_038341348.1	DUF945 domain-containing protein	-
I6H01_RS03705	719986..720465	+	480	WP_038341347.1	antirestriction protein	-
I6H01_RS03710	720481..720957	+	477	WP_001186773.1	RadC family protein	-
I6H01_RS03715	721020..721241	+	222	WP_000692323.1	DUF987 domain-containing protein	-
I6H01_RS03720	721404..721772	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6H01_RS03725	721862..722239	+	378	WP_038341345.1	TA system toxin CbtA family protein	Toxin
I6H01_RS03730	722236..722439	+	204	WP_000761705.1	hypothetical protein	-
I6H01_RS03735	722452..722565	+	114	WP_032140621.1	DUF957 domain-containing protein	-
I6H01_RS03740	723363..723752	+	390	WP_106378927.1	transposase	-
I6H01_RS03745	724207..724389	+	183	WP_024167529.1	ethanolamine utilization protein	-
I6H01_RS03750	724490..724819	-	330	WP_000450409.1	DUF496 family protein	-
I6H01_RS03755	724991..726049	-	1059	WP_001200892.1	FUSC family protein	-
I6H01_RS03760	726247..726720	-	474	WP_001105382.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14123.20 Da
• Isoelectric Point: 7.7531

>T183012 WP_038341345.1 NZ_CP065613:721862-722239 [Escherichia coli]
MKTLPDTHVREVSCCPSLVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVVKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T183012 NZ_CP086625:3497936-3498322 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT183012 WP_001295723.1 NZ_CP065613:721404-721772 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT183012 NZ_CP086625:3498312-3498641 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A377EB25

Antitoxin

• AlphaFold DB: S1NM52