Detailed information of TA system TA183005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 75362..76950
• Replicon: chromosome
• Accession: NZ_CP065613
• Organism: Escherichia coli strain FDAARGOS_944

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A377CJB1
• Locus tag: I6H01_RS00330
• Protein ID: WP_038341703.1
• Coordinates: 75362..76684 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: I6H01_RS00335
• Protein ID: WP_001613070.1
• Coordinates: 76684..76950 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H01_RS00310	70472..71002	-	531	WP_000876770.1	type 1 fimbrial protein	-
I6H01_RS00315	71016..73667	-	2652	WP_038341704.1	fimbrial biogenesis outer membrane usher protein	-
I6H01_RS00320	73709..74419	-	711	WP_001195166.1	fimbria/pilus periplasmic chaperone	-
I6H01_RS00325	74780..75097	-	318	Protein_64	fimbrial protein	-
I6H01_RS00330	75362..76684	-	1323	WP_038341703.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
I6H01_RS00335	76684..76950	-	267	WP_001613070.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
I6H01_RS00340	77161..78747	-	1587	Protein_67	autotransporter outer membrane beta-barrel domain-containing protein	-
I6H01_RS00345	78975..80171	+	1197	WP_115209946.1	IS110 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	78975..80171	1196

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (18-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49308.89 Da
• Isoelectric Point: 8.5823

>T183005 WP_038341703.1 NZ_CP065613:c76684-75362 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK

Nucleotide

• Length: 1323 bp

>T183005 NZ_CP086625:1304907-1305218 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.36 Da
• Isoelectric Point: 4.9632

>AT183005 WP_001613070.1 NZ_CP065613:c76950-76684 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT183005 NZ_CP086625:1305215-1305634 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A377CJB1