Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 35157..35716 Replicon plasmid unnamed2
Accession NZ_CP065612
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name relE Uniprot ID D2AJT2
Locus tag I6H02_RS27085 Protein ID WP_000421262.1
Coordinates 35157..35432 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID A0A0M1UJH5
Locus tag I6H02_RS27090 Protein ID WP_038341659.1
Coordinates 35432..35716 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS27040 30288..30383 - 96 Protein_39 transposase domain-containing protein -
I6H02_RS27045 30419..30724 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB -
I6H02_RS27050 30726..30944 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA -
I6H02_RS27055 31104..31376 + 273 WP_073840987.1 hypothetical protein -
I6H02_RS27060 31357..32565 + 1209 WP_115264363.1 IS91 family transposase -
I6H02_RS27065 32760..33851 - 1092 Protein_44 IS66 family transposase -
I6H02_RS27070 33859..34427 + 569 Protein_45 plasmid segregation protein ParM -
I6H02_RS27075 34430..34781 + 352 Protein_46 plasmid partitioning/stability family protein -
I6H02_RS27080 34888..35025 + 138 WP_000766699.1 hypothetical protein -
I6H02_RS27085 35157..35432 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6H02_RS27090 35432..35716 - 285 WP_038341659.1 ribbon-helix-helix domain-containing protein Antitoxin
I6H02_RS27095 36240..36419 + 180 Protein_50 MFS transporter -
I6H02_RS27100 36469..36678 + 210 Protein_51 peptidoglycan-binding protein -
I6H02_RS27105 36778..36963 - 186 Protein_52 ATP-binding protein -
I6H02_RS27110 37299..37769 + 471 Protein_53 IS1 family transposase -
I6H02_RS27115 37928..38566 + 639 WP_000607008.1 type III secretion system effector OspI -
I6H02_RS27120 38681..38950 + 270 Protein_55 transposase -
I6H02_RS27125 39038..40266 + 1229 WP_181880336.1 IS3-like element IS2 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB / ospE2 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2 1..277227 277227
- inside IScluster/Tn - ospG / ipaH9.8 / ospI 24806..47730 22924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10766.48 Da        Isoelectric Point: 9.9324

>T183002 WP_000421262.1 NZ_CP065612:c35432-35157 [Escherichia coli]
MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T183002 NZ_CP086625:c958017-957643 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10444.88 Da        Isoelectric Point: 5.5648

>AT183002 WP_038341659.1 NZ_CP065612:c35716-35432 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEECNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDHPLPVPR

Download         Length: 285 bp

>AT183002 NZ_CP086625:c958438-958064 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822PVM3

Antitoxin

Source ID Structure
AlphaFold DB A0A0M1UJH5

References