Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 30419..30944 Replicon plasmid unnamed2
Accession NZ_CP065612
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name ccdB Uniprot ID Q326Z8
Locus tag I6H02_RS27045 Protein ID WP_001159860.1
Coordinates 30419..30724 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID Q7BEK0
Locus tag I6H02_RS27050 Protein ID WP_000813626.1
Coordinates 30726..30944 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS27010 26294..26413 - 120 WP_071845702.1 type III effector -
I6H02_RS27015 26463..27160 + 698 Protein_34 IS1 family transposase -
I6H02_RS27020 27175..27660 - 486 WP_038339729.1 protein kinase -
I6H02_RS27025 27689..27915 - 227 Protein_36 type II toxin-antitoxin system RelE/ParE family toxin -
I6H02_RS27030 27912..28181 - 270 WP_000079956.1 hypothetical protein -
I6H02_RS27035 28389..30026 - 1638 WP_115264364.1 T3SS effector E3 ubiquitin-protein ligase IpaH9.8 -
I6H02_RS27040 30288..30383 - 96 Protein_39 transposase domain-containing protein -
I6H02_RS27045 30419..30724 - 306 WP_001159860.1 type II toxin-antitoxin system toxin CcdB Toxin
I6H02_RS27050 30726..30944 - 219 WP_000813626.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
I6H02_RS27055 31104..31376 + 273 WP_073840987.1 hypothetical protein -
I6H02_RS27060 31357..32565 + 1209 WP_115264363.1 IS91 family transposase -
I6H02_RS27065 32760..33851 - 1092 Protein_44 IS66 family transposase -
I6H02_RS27070 33859..34427 + 569 Protein_45 plasmid segregation protein ParM -
I6H02_RS27075 34430..34781 + 352 Protein_46 plasmid partitioning/stability family protein -
I6H02_RS27080 34888..35025 + 138 WP_000766699.1 hypothetical protein -
I6H02_RS27085 35157..35432 - 276 WP_000421262.1 type II toxin-antitoxin system RelE/ParE family toxin -
I6H02_RS27090 35432..35716 - 285 WP_038341659.1 ribbon-helix-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - ospG / ipaH9.8 / ospI / papC / icsA/virG / virA / spa40 / spa29 / spa9 / spa24 / spa33 / spa32 / spa13 / spa47 / spa15 / mxiA / mxiC / mxiD / mxiE / mxiM / mxiL / mxiN / mxiK / mxiJ / mxiI / mxiH / mxiG / ipgF / ipgE / ipgD / icsB / ipgA / ipgB1 / ipgC / ipaB / ipaC / ipaD / ipaA / ipaJ / ospC3 / ospD3/senA / ospC1 / faeI / faeH / faeF / faeE / faeD / faeC / senB / ospE2 / ospE1 / ipaH1.4 / ipaH7.8 / ipaH4.5 / ipgB2 / ospD1 / ospF / ospD2 / ospC2 / ospC4 / ospC4 / ospB / icsP/sopA / nleE / ospC2 1..277227 277227
- inside IScluster/Tn - ospG / ipaH9.8 / ospI 24806..47730 22924


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11736.59 Da        Isoelectric Point: 6.4674

>T183001 WP_001159860.1 NZ_CP065612:c30724-30419 [Escherichia coli]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T183001 NZ_CP086625:c883341-882964 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8333.36 Da        Isoelectric Point: 6.2838

>AT183001 WP_000813626.1 NZ_CP065612:c30944-30726 [Escherichia coli]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT183001 NZ_CP086625:c883799-883431 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTN3

Antitoxin

Source ID Structure
AlphaFold DB Q7BEK0

References