Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3920745..3921363 Replicon chromosome
Accession NZ_CP065611
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6H02_RS20145 Protein ID WP_001291435.1
Coordinates 3920745..3920963 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6H02_RS20150 Protein ID WP_000344800.1
Coordinates 3920989..3921363 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS20120 3916551..3916904 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6H02_RS20125 3916946..3918496 - 1551 WP_001344255.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6H02_RS20130 3918700..3919397 - 698 Protein_3792 IS1 family transposase -
I6H02_RS20135 3919448..3919906 - 459 WP_115264091.1 YlaC family protein -
I6H02_RS20140 3920022..3920573 - 552 WP_000102569.1 maltose O-acetyltransferase -
I6H02_RS20145 3920745..3920963 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6H02_RS20150 3920989..3921363 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6H02_RS20155 3921909..3925058 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
I6H02_RS20160 3925081..3926274 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182997 WP_001291435.1 NZ_CP065611:c3920963-3920745 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T182997 NZ_CP086625:263108-263215 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT182997 WP_000344800.1 NZ_CP065611:c3921363-3920989 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT182997 NZ_CP086625:c263050-262996 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References