Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2390463..2391094 Replicon chromosome
Accession NZ_CP065611
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name hicA Uniprot ID A0A377MSE5
Locus tag I6H02_RS12850 Protein ID WP_001259383.1
Coordinates 2390819..2391094 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag I6H02_RS12845 Protein ID WP_000593555.1
Coordinates 2390463..2390822 (-) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS12820 2386596..2387540 + 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -
I6H02_RS12825 2387537..2388370 + 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
I6H02_RS12830 2388370..2389134 + 765 WP_001136241.1 nickel import ATP-binding protein NikD -
I6H02_RS12835 2389131..2389937 + 807 WP_000173702.1 nickel import ATP-binding protein NikE -
I6H02_RS12840 2389942..2390343 + 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
I6H02_RS12845 2390463..2390822 - 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
I6H02_RS12850 2390819..2391094 - 276 WP_001259383.1 type II toxin-antitoxin system HicA family toxin Toxin
I6H02_RS12855 2391167..2392291 - 1125 WP_115264197.1 ABC transporter permease -
I6H02_RS12860 2392291..2395026 - 2736 WP_115264196.1 ribosome-associated ATPase/putative transporter RbbA -
I6H02_RS12865 2395023..2396090 - 1068 WP_000361498.1 HlyD family efflux transporter periplasmic adaptor subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10209.98 Da        Isoelectric Point: 10.7076

>T182992 WP_001259383.1 NZ_CP065611:c2391094-2390819 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGPRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T182992 NZ_CP086624:c4646652-4646302 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT182992 WP_000593555.1 NZ_CP065611:c2390822-2390463 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT182992 NZ_CP086624:c4646897-4646646 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377MSE5

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References