Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1813133..1813787 Replicon chromosome
Accession NZ_CP065611
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag I6H02_RS09945 Protein ID WP_000244777.1
Coordinates 1813133..1813540 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag I6H02_RS09950 Protein ID WP_000354046.1
Coordinates 1813521..1813787 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS09925 1809091..1810824 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
I6H02_RS09930 1810830..1811540 - 711 WP_032359753.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
I6H02_RS09935 1811564..1812460 - 897 WP_000806634.1 site-specific tyrosine recombinase XerD -
I6H02_RS09940 1812572..1813093 + 522 WP_001055874.1 flavodoxin FldB -
I6H02_RS09945 1813133..1813540 - 408 WP_000244777.1 toxin CptA Toxin
I6H02_RS09950 1813521..1813787 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
I6H02_RS09955 1814030..1815010 + 981 WP_000886095.1 tRNA-modifying protein YgfZ -
I6H02_RS09960 1815206..1815865 - 660 WP_000250274.1 hemolysin III family protein -
I6H02_RS09965 1816029..1816340 - 312 WP_001182947.1 N(4)-acetylcytidine aminohydrolase -
I6H02_RS09970 1816385..1817818 + 1434 WP_001343615.1 6-phospho-beta-glucosidase BglA -
I6H02_RS09975 1817875..1818618 - 744 WP_000951945.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T182990 WP_000244777.1 NZ_CP065611:c1813540-1813133 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T182990 NZ_CP086624:c4153414-4153016 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT182990 WP_000354046.1 NZ_CP065611:c1813787-1813521 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT182990 NZ_CP086624:c4153710-4153417 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References