Detailed information of TA system TA182987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1345593..1346218
• Replicon: chromosome
• Accession: NZ_CP065611
• Organism: Escherichia coli strain FDAARGOS_945

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: I6H02_RS07655
• Protein ID: WP_000911330.1
• Coordinates: 1345593..1345991 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: I6H02_RS07660
• Protein ID: WP_000450524.1
• Coordinates: 1345991..1346218 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H02_RS07635	1341471..1341671	+	201	WP_000383836.1	YpfN family protein	-
I6H02_RS07640	1341781..1342479	-	699	WP_000679796.1	esterase	-
I6H02_RS07645	1342553..1344568	-	2016	WP_000829335.1	tRNA cytosine(34) acetyltransferase TmcA	-
I6H02_RS07650	1344583..1345446	-	864	WP_001267498.1	neutral zinc metallopeptidase	-
I6H02_RS07655	1345593..1345991	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
I6H02_RS07660	1345991..1346218	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
I6H02_RS07665	1346372..1347085	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
I6H02_RS07670	1347298..1348332	-	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
I6H02_RS07675	1348349..1349227	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
I6H02_RS07680	1349373..1349945	+	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
I6H02_RS07685	1349945..1350415	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T182987 WP_000911330.1 NZ_CP065611:c1345991-1345593 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T182987 NZ_CP086624:3470615-3471001 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT182987 WP_000450524.1 NZ_CP065611:c1346218-1345991 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT182987 NZ_CP086624:3470991-3471320 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33