Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) paaR-paaA-parE/-
Location 754356..754840 Replicon chromosome
Accession NZ_CP065611
Organism Escherichia coli strain FDAARGOS_945

Toxin (Protein)


Gene name parE_1 Uniprot ID A0A377EAU1
Locus tag I6H02_RS04810 Protein ID WP_014334020.1
Coordinates 754550..754840 (+) Length 97 a.a.

Antitoxin (Protein)


Gene name paaA Uniprot ID A0A376K4R9
Locus tag I6H02_RS04805 Protein ID WP_005142544.1
Coordinates 754356..754550 (+) Length 65 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H02_RS04765 749476..749892 - 417 WP_005069274.1 hypothetical protein -
I6H02_RS04770 749989..750444 - 456 WP_000335375.1 ead/Ea22-like family protein -
I6H02_RS04775 750431..750736 - 306 WP_001266147.1 DUF4406 domain-containing protein -
I6H02_RS04780 750733..751155 - 423 WP_001151146.1 DUF977 family protein -
I6H02_RS04785 751196..752294 - 1099 Protein_814 DNA-binding protein -
I6H02_RS04790 752374..752832 + 459 WP_073801390.1 hypothetical protein -
I6H02_RS04795 753039..753464 - 426 WP_000693902.1 Rha family transcriptional regulator -
I6H02_RS04800 753836..754336 + 501 WP_001343764.1 helix-turn-helix domain-containing protein -
I6H02_RS04805 754356..754550 + 195 WP_005142544.1 hypothetical protein Antitoxin
I6H02_RS04810 754550..754840 + 291 WP_014334020.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
I6H02_RS04815 755116..755268 + 153 WP_115264555.1 DUF1391 family protein -
I6H02_RS04820 755280..755621 + 342 WP_115264556.1 hypothetical protein -
I6H02_RS04830 757000..757446 - 447 WP_000373330.1 hypothetical protein -
I6H02_RS04835 757846..759036 - 1191 WP_001111632.1 IS4-like element ISEc60 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - ipaH9.8 717396..793912 76516


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-91)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11062.93 Da        Isoelectric Point: 10.2013

>T182986 WP_014334020.1 NZ_CP065611:754550-754840 [Escherichia coli]
MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLTEHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIE
VVNIVHSRRQYPGKNS

Download         Length: 291 bp

>T182986 NZ_CP086624:3215093-3215587 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin


Download         Length: 65 a.a.        Molecular weight: 7097.98 Da        Isoelectric Point: 4.1759

>AT182986 WP_005142544.1 NZ_CP065611:754356-754550 [Escherichia coli]
MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDEVMAEMEQIIAQIAAATHRGE

Download         Length: 195 bp

>AT182986 NZ_CP086624:3214803-3215096 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377EAU1

Antitoxin

Source ID Structure
AlphaFold DB A0A376K4R9

References