Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3592752..3593370 Replicon chromosome
Accession NZ_CP065607
Organism Escherichia coli strain FDAARGOS_946

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag I6H03_RS17575 Protein ID WP_001291435.1
Coordinates 3592752..3592970 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag I6H03_RS17580 Protein ID WP_000344800.1
Coordinates 3592996..3593370 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6H03_RS17540 3588039..3588611 + 573 WP_000779831.1 YbaY family lipoprotein -
I6H03_RS17545 3588644..3588955 - 312 WP_000409911.1 MGMT family protein -
I6H03_RS17555 3589334..3589687 + 354 WP_000878140.1 DUF1428 domain-containing protein -
I6H03_RS17560 3589729..3591279 - 1551 WP_001301851.1 cyclic-guanylate-specific phosphodiesterase PdeB -
I6H03_RS17565 3591443..3591913 - 471 WP_000136192.1 YlaC family protein -
I6H03_RS17570 3592029..3592580 - 552 WP_000102564.1 maltose O-acetyltransferase -
I6H03_RS17575 3592752..3592970 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
I6H03_RS17580 3592996..3593370 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
I6H03_RS17585 3593916..3597065 - 3150 WP_001132468.1 multidrug efflux RND transporter permease subunit -
I6H03_RS17590 3597088..3598281 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182969 WP_001291435.1 NZ_CP065607:c3592970-3592752 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T182969 NZ_CP086623:c5045523-5045212 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTCACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT182969 WP_000344800.1 NZ_CP065607:c3593370-3592996 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT182969 NZ_CP086623:c5045211-5044921 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References