Detailed information of TA system TA182966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3110721..3111271
• Replicon: chromosome
• Accession: NZ_CP065607
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: I6H03_RS15375
• Protein ID: WP_001160975.1
• Coordinates: 3110957..3111271 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: I6H03_RS15370
• Protein ID: WP_000125566.1
• Coordinates: 3110721..3110954 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS15345	3105795..3106082	+	288	WP_000203741.1	ferredoxin-like protein FixX	-
I6H03_RS15350	3106140..3107471	+	1332	WP_001183177.1	MFS transporter	-
I6H03_RS15355	3107579..3108109	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
I6H03_RS15360	3108102..3109964	+	1863	WP_000377186.1	glutathione-regulated potassium-efflux system protein KefC	-
I6H03_RS15365	3110156..3110635	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
I6H03_RS15370	3110721..3110954	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
I6H03_RS15375	3110957..3111271	+	315	WP_001160975.1	CcdB family protein	Toxin
I6H03_RS15380	3111268..3112116	-	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
I6H03_RS15385	3112123..3112500	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
I6H03_RS15390	3112503..3113324	-	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
I6H03_RS15395	3113321..3114310	-	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
I6H03_RS15400	3114310..3115596	-	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T182966 WP_001160975.1 NZ_CP065607:3110957-3111271 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T182966 NZ_CP086623:c4498648-4498361 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT182966 WP_000125566.1 NZ_CP065607:3110721-3110954 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT182966 NZ_CP086623:c4498374-4498072 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9