Detailed information of TA system TA182965

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2912513..2913347
• Replicon: chromosome
• Accession: NZ_CP065607
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: I6H03_RS14540
• Protein ID: WP_000854819.1
• Coordinates: 2912970..2913347 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: F4THW5
• Locus tag: I6H03_RS14535
• Protein ID: WP_001313071.1
• Coordinates: 2912513..2912881 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS14505	2909432..2909887	+	456	WP_000581504.1	hypothetical protein	-
I6H03_RS14510	2909966..2910199	+	234	WP_001119729.1	DUF905 family protein	-
I6H03_RS14515	2910299..2911117	+	819	WP_001234649.1	DUF945 domain-containing protein	-
I6H03_RS14520	2911172..2911657	+	486	WP_000849584.1	antirestriction protein	-
I6H03_RS14525	2911673..2912149	+	477	WP_001186726.1	RadC family protein	-
I6H03_RS14530	2912212..2912433	+	222	WP_000692301.1	DUF987 domain-containing protein	-
I6H03_RS14535	2912513..2912881	+	369	WP_001313071.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
I6H03_RS14540	2912970..2913347	+	378	WP_000854819.1	type IV toxin-antitoxin system toxin CbtA	Toxin
I6H03_RS14545	2913344..2913832	+	489	WP_000761721.1	hypothetical protein	-
I6H03_RS14550	2913844..2914041	+	198	WP_000839278.1	DUF957 domain-containing protein	-
I6H03_RS14555	2914126..2914278	+	153	WP_001280446.1	hypothetical protein	-
I6H03_RS14560	2914742..2915569	+	828	WP_001359437.1	integrase arm-type DNA-binding domain-containing protein	-
I6H03_RS14565	2915644..2915831	+	188	Protein_2745	transposase	-
I6H03_RS14570	2916029..2916880	-	852	WP_000168588.1	HNH endonuclease	-
I6H03_RS14575	2916954..2917196	-	243	Protein_2747	N-acetylneuraminic acid mutarotase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	2890038..2924465	34427

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14160.18 Da
• Isoelectric Point: 7.8523

>T182965 WP_000854819.1 NZ_CP065607:2912970-2913347 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T182965 NZ_CP086623:c4160779-4160381 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13498.23 Da
• Isoelectric Point: 5.8696

>AT182965 WP_001313071.1 NZ_CP065607:2912513-2912881 [Escherichia coli]
VSDTLSGTTHPDDNNSHPWWGLPCTVTPCFGACLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT182965 NZ_CP086623:c4161075-4160782 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1M1L2Z3