Detailed information of TA system TA182961

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 1887780..1888580
• Replicon: chromosome
• Accession: NZ_CP065607
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A891SNC1
• Locus tag: I6H03_RS09775
• Protein ID: WP_000342448.1
• Coordinates: 1887780..1888307 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4NNI1
• Locus tag: I6H03_RS09780
• Protein ID: WP_001277108.1
• Coordinates: 1888314..1888580 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS09750	1882855..1883622	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
I6H03_RS09755	1883619..1884896	-	1278	WP_000803797.1	branched chain amino acid ABC transporter permease LivM	-
I6H03_RS09760	1884893..1885819	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
I6H03_RS09765	1885867..1886976	-	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
I6H03_RS09770	1887400..1887783	+	384	WP_000778769.1	aspartate 1-decarboxylase autocleavage activator PanM	-
I6H03_RS09775	1887780..1888307	-	528	WP_000342448.1	GNAT family N-acetyltransferase	Toxin
I6H03_RS09780	1888314..1888580	-	267	WP_001277108.1	DUF1778 domain-containing protein	Antitoxin
I6H03_RS09785	1888730..1889833	-	1104	WP_001021984.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
I6H03_RS09790	1890104..1890958	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
I6H03_RS09795	1891203..1892261	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
I6H03_RS09800	1892254..1892922	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19692.68 Da
• Isoelectric Point: 7.3232

>T182961 WP_000342448.1 NZ_CP065607:c1888307-1887780 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T182961 NZ_CP086623:3231936-3232430 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9910.31 Da
• Isoelectric Point: 10.0863

>AT182961 WP_001277108.1 NZ_CP065607:c1888580-1888314 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT182961 NZ_CP086623:3231646-3231939 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• PDB: 6GTS

Antitoxin

• AlphaFold DB: A0A829CN24