Detailed information of TA system TA182945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 3381..3916
• Replicon: plasmid unnamed1
• Accession: NZ_CP065606
• Organism: Escherichia coli strain FDAARGOS_946

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: I6H03_RS00025
• Protein ID: WP_001270421.1
• Coordinates: 3629..3916 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: I6H03_RS00020
• Protein ID: WP_001132893.1
• Coordinates: 3381..3632 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H03_RS00010	828..1790	-	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
I6H03_RS00015	1787..2992	-	1206	WP_000817637.1	AAA family ATPase	-
I6H03_RS00020	3381..3632	+	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
I6H03_RS00025	3629..3916	+	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
I6H03_RS00030	3918..4323	+	406	Protein_5	DDE-type integrase/transposase/recombinase	-
I6H03_RS00035	4412..4561	+	150	WP_001483821.1	hypothetical protein	-
I6H03_RS00040	4969..5946	+	978	WP_000361616.1	RepB family plasmid replication initiator protein	-
I6H03_RS00045	6230..6970	-	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
I6H03_RS00050	7094..7234	-	141	Protein_9	serine/threonine protein kinase	-
I6H03_RS00055	7285..7446	+	162	Protein_10	epoxyqueuosine reductase QueH	-
I6H03_RS00060	7757..8131	-	375	WP_001368887.1	hypothetical protein	-
I6H03_RS00065	8334..8513	+	180	WP_000526857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	nleA/espI / exeG / exeE / stcE	1..66104	66104

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T182945 WP_001270421.1 NZ_CP065606:3629-3916 [Escherichia coli]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T182945 NZ_CP086623:c72958-72584 [Escherichia coli]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT182945 WP_001132893.1 NZ_CP065606:3381-3632 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT182945 NZ_CP086623:c73416-73048 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724