Detailed information of TA system TA182937

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 105511..106283
• Replicon: plasmid unnamed2
• Accession: NZ_CP065603
• Organism: Burkholderia glumae strain FDAARGOS_949

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: I6H06_RS29750
• Protein ID: WP_080942736.1
• Coordinates: 105511..106005 (-)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: A0A2J9HHX2
• Locus tag: I6H06_RS29755
• Protein ID: WP_045678699.1
• Coordinates: 106002..106283 (-)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H06_RS29740	101937..104858	+	2922	WP_017432540.1	conjugative relaxase	-
I6H06_RS29745	104948..105418	-	471	WP_080942737.1	ProQ activator of osmoprotectant transporter prop	-
I6H06_RS29750	105511..106005	-	495	WP_080942736.1	GNAT family N-acetyltransferase	Toxin
I6H06_RS29755	106002..106283	-	282	WP_045678699.1	DUF1778 domain-containing protein	Antitoxin
I6H06_RS29760	106471..106928	+	458	Protein_79	transposase	-
I6H06_RS29765	107179..107460	+	282	WP_042967384.1	virulence factor	-
I6H06_RS29770	107617..108696	-	1080	WP_017432519.1	MBL fold metallo-hydrolase	-
I6H06_RS29775	108743..109462	-	720	WP_045678698.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..196019	196019

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-84)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17493.08 Da
• Isoelectric Point: 8.6141

>T182937 WP_080942736.1 NZ_CP065603:c106005-105511 [Burkholderia glumae]
MTGWLAPEPLGDAHGLDDFDSGVASLDTWLKRRAFANQRSGASRTFVATRDGRVGAYYALASGAVTLEVAPGRFRRNMPD
PIPVVVLGRLAVDRAHQGNGVGRALVRDVGLRVLHAAAAIGIRGLIVHALTDSAKAFYERVGFEASPIDPMLLLITLADL
EHAL

Nucleotide

• Length: 495 bp

>T182937 NZ_CP086622:3501364-3501750 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10112.39 Da
• Isoelectric Point: 7.0104

>AT182937 WP_045678699.1 NZ_CP065603:c106283-106002 [Burkholderia glumae]
MTSTTPRDTLNLRIRPDDRSLIDRAAAAAHKTRTDFVLDAARAAAEQTLLDQVVLSAEPQAYAAFLARLDAPAQPNAALR
QTMQTRAPWDPAA

Nucleotide

• Length: 282 bp

>AT182937 NZ_CP086622:3501740-3502069 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J9HHX2