Detailed information of TA system TA182936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 17046..17641
• Replicon: plasmid unnamed1
• Accession: NZ_CP065602
• Organism: Burkholderia glumae strain FDAARGOS_949

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: I6H06_RS28685
• Protein ID: WP_012734107.1
• Coordinates: 17291..17641 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: I6H06_RS28680
• Protein ID: WP_012734108.1
• Coordinates: 17046..17297 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6H06_RS28645	12315..12503	+	189	WP_017432455.1	hypothetical protein	-
I6H06_RS28650	13255..13539	+	285	WP_017432347.1	hypothetical protein	-
I6H06_RS28655	13553..13795	+	243	WP_124837682.1	hypothetical protein	-
I6H06_RS28660	13792..14037	+	246	WP_127913916.1	hypothetical protein	-
I6H06_RS28665	14094..14435	+	342	WP_012732710.1	hypothetical protein	-
I6H06_RS28670	14438..16273	+	1836	WP_045678669.1	site-specific integrase	-
I6H06_RS28675	16358..16783	+	426	WP_012734109.1	hypothetical protein	-
I6H06_RS28680	17046..17297	+	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I6H06_RS28685	17291..17641	+	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
I6H06_RS28690	17700..17894	+	195	WP_124837685.1	hypothetical protein	-
I6H06_RS28695	18044..18265	+	222	WP_017433768.1	hypothetical protein	-
I6H06_RS28700	18488..18952	+	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
I6H06_RS28705	19254..21641	+	2388	WP_045678668.1	TonB-dependent receptor	-
I6H06_RS28710	21631..22419	+	789	WP_012734104.1	energy transducer TonB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..144540	144540

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T182936 WP_012734107.1 NZ_CP065602:17291-17641 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T182936 NZ_CP086622:3246261-3246755 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT182936 WP_012734108.1 NZ_CP065602:17046-17297 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT182936 NZ_CP086622:3245971-3246264 [Escherichia coli]
ATGCCAGCAGCCAATAACATTCCAACCAGGCGCGAATCTTTAAATTTGCGCATAAAACCCGCAGAACGCGATCTTATTGA
CCGGGCCGCTAAAGCCCGAGGAAAAAATCGCACCGAATTTGTCCTTGAGGCCGCCAGAGCCGCAGCTGAAGAGGCGCTCA
TTGAGCAGAGAATTTTCAGGATCGATTCTGATGATTACCAGCTATTTTTGAATCGTCTGGATCAGCCCCCCGCGCCCAAT
GCTGCATTACGCAAAACCATGCAAACACCCGCGCCGTGGGAGCAAAAAAAATGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5