Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 541691..542327 Replicon chromosome
Accession NZ_CP065591
Organism Bacillus altitudinis strain 1817

Toxin (Protein)


Gene name mazF Uniprot ID K2MHT4
Locus tag I4W80_RS02650 Protein ID WP_003214169.1
Coordinates 541977..542327 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W8QJ31
Locus tag I4W80_RS02645 Protein ID WP_003214273.1
Coordinates 541691..541972 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4W80_RS02625 537845..538450 - 606 WP_007496491.1 rhomboid family intramembrane serine protease -
I4W80_RS02630 538545..538910 + 366 WP_019744212.1 holo-ACP synthase -
I4W80_RS02635 539071..540087 + 1017 WP_007496489.1 outer membrane lipoprotein-sorting protein -
I4W80_RS02640 540216..541397 + 1182 WP_206115996.1 alanine racemase -
I4W80_RS02645 541691..541972 + 282 WP_003214273.1 hypothetical protein Antitoxin
I4W80_RS02650 541977..542327 + 351 WP_003214169.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
I4W80_RS02655 542442..543272 + 831 WP_017358395.1 STAS domain-containing protein -
I4W80_RS02660 543277..543645 + 369 WP_003214235.1 RsbT antagonist protein RsbS -
I4W80_RS02665 543648..544049 + 402 WP_003214085.1 anti-sigma regulatory factor -
I4W80_RS02670 544060..545067 + 1008 WP_017358394.1 PP2C family protein-serine/threonine phosphatase -
I4W80_RS02675 545127..545456 + 330 WP_017358393.1 anti-sigma factor antagonist -
I4W80_RS02680 545453..545941 + 489 WP_007496471.1 anti-sigma B factor RsbW -
I4W80_RS02685 545907..546695 + 789 WP_012009000.1 RNA polymerase sigma factor SigB -
I4W80_RS02690 546695..547294 + 600 WP_008346897.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12976.99 Da        Isoelectric Point: 5.1663

>T182933 WP_003214169.1 NZ_CP065591:541977-542327 [Bacillus altitudinis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEINAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVDEALQVSLALIDF

Download         Length: 351 bp

>T182933 NZ_CP086622:2732975-2734297 [Escherichia coli]
ATGCCTAAACTTGTCACTTGGATGAACAACCAGCGGGTAGGCGAGTTAACGAAGTTAGCCAATGGCGCGCACACCTTTAA
GTATGCACCGGAGTGGTTAGCCAACCGTTATGCCAGACCGTTATCACTTTCGCTGCCATTGCAGAGGGGGAATATCACCT
CTGATGCCGTATTTAACTTCTTCGATAACCTGTTACCCGATAGCCCGATTGTACGTGACCGGATCGTTAAACGTTATCAT
GCCAAATCCAGACAACCGTTTGATTTATTGTCAGAAATAGGGCGAGACAGCGTTGGTGCCGTGACGTTAATACCCGAAGA
TGAAACCGTAACGCATCCGATAATGGCATGGGAAAAGCTTACTGAAGCCAGACTTGAAGAAGTATTAACGGCTTATAAAG
CAGATATCCCTCTGGGCATGATTAGAGAAGAAAATGACTTTCGCATCTCGGTTGCTGGCGCGCAGGAGAAGACAGCGCTG
CTCAGAATAGGCAATGACTGGTGCATTCCGAAAGGAATAACGCCGACGACGCACATCATTAAATTACCGATTGGCGAAAT
CAGGCAGCCCAATGCGACGCTCGATCTCAGCCAAAGCGTTGATAATGAGTATTACTGTCTGCTGCTGGCGAAAGAACTTG
GGTTGAATGTGCCGGACGCAGAAATCATTAAAGCGGGGAATGTGCGCGCGTTAGCGGTCGAACGTTTTGACAGGCGTTGG
AATGCTGAGCGAACGGTTTTACTTCGCTTGCCACAGGAGGATATGTGTCAGACATTCGGTTTACCTTCATCGGTGAAATA
TGAATCAGATGGAGGCCCAGGCATCGCGCGGATCATGGCTTTTTTGATGGGGTCCAGCGAGGCGCTGAAAGATCGCTATG
ATTTTATGAAGTTTCAGGTGTTCCAGTGGTTGATTGGCGCAACGGATGGCCATGCAAAAAACTTCTCCGTATTTATTCAG
GCTGGCGGCAGTTATCGACTCACGCCATTTTACGACATCATTTCAGCATTTCCGGTATTGGGTGGCGCAGGAATACACAT
CAGCGATCTCAAACTGGCAATGGGGGTTAACGCATCCAAAGGCAAAAAAACGGCAATCGATAAAATTTATCCGCGACATT
TTCTGGCGACAGCAAAGGTGCTGAAATTTCCGGAAGTGCAGATGCATGAAATCCTGAGTGACTTTGCCAGAATGATTCCG
GCAGCACTGGATAACGTGAAGACTTCATTACCGACAGATTTTCCGGAGAACGTGGTGACGGCAGTTGAAACCAATGTGTT
GAGGTTGCACGGCCGGTTAAGCCGAGAATACGGTATTAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10864.25 Da        Isoelectric Point: 5.2726

>AT182933 WP_003214273.1 NZ_CP065591:541691-541972 [Bacillus altitudinis]
MSESSATNEMKIRLPEALMSEIERVAEQDKVTRNELIYRATKTYIRERNIRQNRESMRRGYMEMAKINLNISSEAQYAEF
EAENTVERLVSGG

Download         Length: 282 bp

>AT182933 NZ_CP086622:2732709-2732975 [Escherichia coli]
ATGATGAGCTTTCAGAAGATCTATAGCCCAACGCAATTAGCGAATGCAATGAAACTGGTTCGTCAGCAAAATGGCTGGAC
GCAGAGCGAGCTGGCGAAAAAAATCGGCATTAAGCAGGCGACAATTTCCAATTTCGAAAACAACCCTGACAATACCACGC
TGACGACATTTTTTAAGATTTTACAGTCGCTTGAACTCTCAATGACGCTATGCGACACGAAAAATGCCTCGCCAGAATCA
ACAGAACAGCAAGATCTGGAGTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB K2MHT4

Antitoxin

Source ID Structure
AlphaFold DB A0A081L854

References