Detailed information of TA system TA182920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 32569..33239
• Replicon: plasmid unnamed1
• Accession: NZ_CP065555
• Organism: Klebsiella pneumoniae strain FK 6768

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: I7S93_RS27290
• Protein ID: WP_004213072.1
• Coordinates: 32569..33012 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: I7S93_RS27295
• Protein ID: WP_004213073.1
• Coordinates: 33009..33239 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I7S93_RS27255 (I7S93_27185)	27980..28255	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
I7S93_RS27260 (I7S93_27190)	28318..28809	+	492	WP_011251327.1	DM13 domain-containing protein	-
I7S93_RS27265 (I7S93_27195)	28858..29778	+	921	WP_004213934.1	DUF1471 domain-containing protein	-
I7S93_RS27270 (I7S93_27200)	29869..30272	+	404	Protein_35	GAF domain-containing protein	-
I7S93_RS27275 (I7S93_27205)	30790..31425	-	636	Protein_36	mucoid phenotype regulator RmpA2	-
I7S93_RS27280 (I7S93_27210)	31842..32146	+	305	Protein_37	transposase	-
I7S93_RS27285	32169..32420	-	252	WP_186987481.1	hypothetical protein	-
I7S93_RS27290 (I7S93_27220)	32569..33012	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
I7S93_RS27295 (I7S93_27225)	33009..33239	-	231	WP_004213073.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
I7S93_RS27300 (I7S93_27230)	33847..34980	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
I7S93_RS27305 (I7S93_27235)	34996..35289	+	294	WP_004213076.1	hypothetical protein	-
I7S93_RS27310 (I7S93_27240)	35279..35485	-	207	WP_004213077.1	hypothetical protein	-
I7S93_RS27315 (I7S93_27245)	36457..37278	+	822	WP_004213562.1	hypothetical protein	-
I7S93_RS27320 (I7S93_27250)	37275..38054	+	780	WP_004213560.1	site-specific integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / iroN / rmpA	1..219459	219459

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T182920 WP_004213072.1 NZ_CP065555:c33012-32569 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T182920 NZ_CP086621:72314-72619 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT182920 WP_004213073.1 NZ_CP065555:c33239-33009 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT182920 NZ_CP086621:72094-72312 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGTCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620