Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5401851..5402476 Replicon chromosome
Accession NZ_CP065554
Organism Klebsiella pneumoniae strain FK 6768

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag I7S93_RS26730 Protein ID WP_002882817.1
Coordinates 5401851..5402234 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag I7S93_RS26735 Protein ID WP_004150355.1
Coordinates 5402234..5402476 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7S93_RS26715 (I7S93_26650) 5399217..5400119 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
I7S93_RS26720 (I7S93_26655) 5400116..5400751 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
I7S93_RS26725 (I7S93_26660) 5400748..5401677 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
I7S93_RS26730 (I7S93_26665) 5401851..5402234 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
I7S93_RS26735 (I7S93_26670) 5402234..5402476 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
I7S93_RS26740 (I7S93_26675) 5402681..5403598 + 918 WP_002882812.1 alpha/beta hydrolase -
I7S93_RS26745 (I7S93_26680) 5403612..5404553 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
I7S93_RS26750 (I7S93_26685) 5404598..5405035 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
I7S93_RS26755 (I7S93_26690) 5405032..5405892 - 861 WP_002882807.1 virulence factor BrkB family protein -
I7S93_RS26760 (I7S93_26695) 5405886..5406485 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T182919 WP_002882817.1 NZ_CP065554:c5402234-5401851 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T182919 NZ_CP086621:62510-62785 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGTCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGAGAACAGTTGTTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAAATTAATGGCCAGACTATTTAT
GTATTGCGTCTATGGCACACACGAGAAAACAGGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT182919 WP_004150355.1 NZ_CP065554:c5402476-5402234 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT182919 NZ_CP086621:62226-62510 [Escherichia coli]
ATGCAAATGAAAAACAATGCCGCACAAGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGTTCCCGGGGCTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
TCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References