Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4203088..4203707 Replicon chromosome
Accession NZ_CP065554
Organism Klebsiella pneumoniae strain FK 6768

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag I7S93_RS20975 Protein ID WP_002892050.1
Coordinates 4203489..4203707 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag I7S93_RS20970 Protein ID WP_002892066.1
Coordinates 4203088..4203462 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7S93_RS20960 (I7S93_20905) 4198240..4199433 + 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
I7S93_RS20965 (I7S93_20910) 4199456..4202602 + 3147 WP_020326861.1 multidrug efflux RND transporter permease subunit AcrB -
I7S93_RS20970 (I7S93_20915) 4203088..4203462 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
I7S93_RS20975 (I7S93_20920) 4203489..4203707 + 219 WP_002892050.1 HHA domain-containing protein Toxin
I7S93_RS20980 (I7S93_20925) 4203866..4204432 + 567 WP_002892030.1 maltose O-acetyltransferase -
I7S93_RS20985 (I7S93_20930) 4204404..4204544 - 141 WP_004147370.1 hypothetical protein -
I7S93_RS20990 (I7S93_20935) 4204565..4205035 + 471 WP_002892026.1 YlaC family protein -
I7S93_RS20995 (I7S93_20940) 4205010..4206461 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
I7S93_RS21000 (I7S93_20945) 4206562..4207260 + 699 WP_002892021.1 GNAT family protein -
I7S93_RS21005 (I7S93_20950) 4207257..4207397 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
I7S93_RS21010 (I7S93_20955) 4207397..4207660 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T182915 WP_002892050.1 NZ_CP065554:4203489-4203707 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T182915 NZ_CP086620:c4481828-4481541 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT182915 WP_002892066.1 NZ_CP065554:4203088-4203462 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT182915 NZ_CP086620:c4481554-4481252 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References