Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 517120..517691 Replicon chromosome
Accession NZ_CP065547
Organism Enterococcus faecium strain Dallas

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag I7089_RS02465 Protein ID WP_002286801.1
Coordinates 517350..517691 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag I7089_RS02460 Protein ID WP_002323011.1
Coordinates 517120..517350 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I7089_RS02430 512253..513584 + 1332 WP_002286818.1 FAD-containing oxidoreductase -
I7089_RS02435 513606..514232 + 627 WP_002286816.1 cysteine hydrolase -
I7089_RS02440 514415..514996 + 582 WP_010729547.1 TetR/AcrR family transcriptional regulator -
I7089_RS02445 515728..516075 + 348 WP_002286808.1 SOS response-associated peptidase family protein -
I7089_RS02450 516051..516290 + 240 WP_002321938.1 SOS response-associated peptidase family protein -
I7089_RS02455 516495..516833 - 339 WP_002286804.1 hypothetical protein -
I7089_RS02460 517120..517350 + 231 WP_002323011.1 hypothetical protein Antitoxin
I7089_RS02465 517350..517691 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I7089_RS02470 518541..518726 + 186 WP_002304207.1 hypothetical protein -
I7089_RS02475 519231..519425 + 195 WP_002297028.1 hypothetical protein -
I7089_RS02480 519583..519865 + 283 Protein_493 transposase -
I7089_RS02485 520109..520939 - 831 WP_002286789.1 manganese catalase family protein -
I7089_RS02490 521147..522481 + 1335 WP_002286788.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T182897 WP_002286801.1 NZ_CP065547:517350-517691 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T182897 NZ_CP086620:262820-262927 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT182897 WP_002323011.1 NZ_CP065547:517120-517350 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT182897 NZ_CP086620:c262771-262708 [Escherichia coli]
GTCTAGATGCAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References