Detailed information of TA system TA182895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1223299..1224216
• Replicon: chromosome
• Accession: NZ_CP065539
• Organism: Bacillus amyloliquefaciens strain DGL1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: I5367_RS06420
• Protein ID: WP_012117368.1
• Coordinates: 1223470..1224216 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: I5367_RS06415
• Protein ID: WP_003154807.1
• Coordinates: 1223299..1223469 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5367_RS06370	1218433..1218945	+	513	WP_012117362.1	sigma-70 family RNA polymerase sigma factor	-
I5367_RS06375	1219058..1219279	+	222	Protein_1194	terminase	-
I5367_RS06380	1219688..1220155	+	468	WP_077411063.1	hypothetical protein	-
I5367_RS06385	1220168..1220539	+	372	WP_012117365.1	hypothetical protein	-
I5367_RS06390	1220544..1220741	+	198	WP_012117366.1	XkdX family protein	-
I5367_RS06395	1220798..1221559	+	762	WP_012117367.1	hypothetical protein	-
I5367_RS06400	1221611..1221874	+	264	WP_003154815.1	hemolysin XhlA family protein	-
I5367_RS06405	1221888..1222151	+	264	WP_003154813.1	phage holin	-
I5367_RS06410	1222165..1223043	+	879	WP_007407257.1	N-acetylmuramoyl-L-alanine amidase	-
I5367_RS06415	1223299..1223469	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
I5367_RS06420	1223470..1224216	-	747	WP_012117368.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
I5367_RS06425	1224321..1225319	-	999	WP_007407255.1	inorganic phosphate transporter	-
I5367_RS06430	1225332..1225949	-	618	WP_003154804.1	DUF47 domain-containing protein	-
I5367_RS06435	1226235..1227551	-	1317	WP_007610842.1	amino acid permease	-
I5367_RS06440	1227874..1228824	+	951	WP_012117369.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1210955..1281503	70548

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29119.61 Da
• Isoelectric Point: 4.7003

>T182895 WP_012117368.1 NZ_CP065539:c1224216-1223470 [Bacillus amyloliquefaciens]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAEWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQADKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T182895 NZ_CP086620:206651-206836 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT182895 WP_003154807.1 NZ_CP065539:c1223469-1223299 [Bacillus amyloliquefaciens]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT182895 NZ_CP086620:206853-207263 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGTGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Antitoxin

• AlphaFold DB: I2HQ14