Detailed information of TA system TA182894

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 488596..489233
• Replicon: chromosome
• Accession: NZ_CP065539
• Organism: Bacillus amyloliquefaciens strain DGL1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: I5367_RS02490
• Protein ID: WP_003156187.1
• Coordinates: 488883..489233 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: I5367_RS02485
• Protein ID: WP_003156188.1
• Coordinates: 488596..488877 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5367_RS02465	484961..485560	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
I5367_RS02470	485653..486018	+	366	WP_012116869.1	holo-ACP synthase	-
I5367_RS02475	486183..487190	+	1008	WP_007410230.1	outer membrane lipoprotein carrier protein LolA	-
I5367_RS02480	487307..488476	+	1170	WP_012116870.1	alanine racemase	-
I5367_RS02485	488596..488877	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
I5367_RS02490	488883..489233	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
I5367_RS02495	489351..490172	+	822	WP_003156182.1	STAS domain-containing protein	-
I5367_RS02500	490177..490542	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
I5367_RS02505	490545..490946	+	402	WP_003156178.1	anti-sigma regulatory factor	-
I5367_RS02510	490958..491965	+	1008	WP_007410233.1	PP2C family protein-serine/threonine phosphatase	-
I5367_RS02515	492029..492358	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
I5367_RS02520	492355..492837	+	483	WP_007609591.1	anti-sigma B factor RsbW	-
I5367_RS02525	492803..493591	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
I5367_RS02530	493591..494193	+	603	WP_012116871.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T182894 WP_003156187.1 NZ_CP065539:488883-489233 [Bacillus amyloliquefaciens]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T182894 NZ_CP086620:c72958-72584 [Escherichia coli]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT182894 WP_003156188.1 NZ_CP065539:488596-488877 [Bacillus amyloliquefaciens]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT182894 NZ_CP086620:c73416-73048 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5