Detailed information of TA system TA182893

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 70883..72020
• Replicon: plasmid pEDU98D1
• Accession: NZ_CP065536
• Organism: Enterococcus durans strain KCTC 13289

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: I4Q39_RS14395
• Protein ID: WP_016177608.1
• Coordinates: 71157..72020 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: I4Q39_RS14390
• Protein ID: WP_000301765.1
• Coordinates: 70883..71155 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I4Q39_RS14360	66229..66837	+	609	WP_016177603.1	recombinase family protein	-
I4Q39_RS14365	66925..67410	+	486	WP_016177604.1	hypothetical protein	-
I4Q39_RS14370	67417..68112	+	696	WP_082705217.1	topoisomerase I	-
I4Q39_RS14375	68097..69560	+	1464	Protein_76	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
I4Q39_RS14380	69663..70559	+	897	WP_016177606.1	ParA family protein	-
I4Q39_RS14385	70657..70866	+	210	WP_016177607.1	hypothetical protein	-
I4Q39_RS14390	70883..71155	+	273	WP_000301765.1	antitoxin	Antitoxin
I4Q39_RS14395	71157..72020	+	864	WP_016177608.1	zeta toxin family protein	Toxin
I4Q39_RS14400	72048..72257	+	210	WP_016177609.1	hypothetical protein	-
I4Q39_RS14405	72695..73012	+	318	WP_002326830.1	hypothetical protein	-
I4Q39_RS14410	74607..74816	+	210	WP_060789721.1	DUF4314 domain-containing protein	-
I4Q39_RS14415	74846..75037	+	192	WP_060789722.1	hypothetical protein	-
I4Q39_RS14420	75070..76029	-	960	WP_002322607.1	IS30-like element IS6770 family transposase	-
I4Q39_RS14425	76540..76842	-	303	WP_060789796.1	helix-turn-helix transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	ClpL	-	1..174669	174669

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32477.05 Da
• Isoelectric Point: 6.6610

>T182893 WP_016177608.1 NZ_CP065536:71157-72020 [Enterococcus durans]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKIPKLPGL

Nucleotide

• Length: 864 bp

>T182893 NZ_CP086619:67463-67768 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT182893 WP_000301765.1 NZ_CP065536:70883-71155 [Enterococcus durans]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT182893 NZ_CP086619:67243-67461 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3