Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 70883..72020 Replicon plasmid pEDU98D1
Accession NZ_CP065536
Organism Enterococcus durans strain KCTC 13289

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag I4Q39_RS14395 Protein ID WP_016177608.1
Coordinates 71157..72020 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag I4Q39_RS14390 Protein ID WP_000301765.1
Coordinates 70883..71155 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I4Q39_RS14360 66229..66837 + 609 WP_016177603.1 recombinase family protein -
I4Q39_RS14365 66925..67410 + 486 WP_016177604.1 hypothetical protein -
I4Q39_RS14370 67417..68112 + 696 WP_082705217.1 topoisomerase I -
I4Q39_RS14375 68097..69560 + 1464 Protein_76 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
I4Q39_RS14380 69663..70559 + 897 WP_016177606.1 ParA family protein -
I4Q39_RS14385 70657..70866 + 210 WP_016177607.1 hypothetical protein -
I4Q39_RS14390 70883..71155 + 273 WP_000301765.1 antitoxin Antitoxin
I4Q39_RS14395 71157..72020 + 864 WP_016177608.1 zeta toxin family protein Toxin
I4Q39_RS14400 72048..72257 + 210 WP_016177609.1 hypothetical protein -
I4Q39_RS14405 72695..73012 + 318 WP_002326830.1 hypothetical protein -
I4Q39_RS14410 74607..74816 + 210 WP_060789721.1 DUF4314 domain-containing protein -
I4Q39_RS14415 74846..75037 + 192 WP_060789722.1 hypothetical protein -
I4Q39_RS14420 75070..76029 - 960 WP_002322607.1 IS30-like element IS6770 family transposase -
I4Q39_RS14425 76540..76842 - 303 WP_060789796.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ClpL - 1..174669 174669


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32477.05 Da        Isoelectric Point: 6.6610

>T182893 WP_016177608.1 NZ_CP065536:71157-72020 [Enterococcus durans]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKIPKLPGL

Download         Length: 864 bp

>T182893 NZ_CP086619:67463-67768 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT182893 WP_000301765.1 NZ_CP065536:70883-71155 [Enterococcus durans]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT182893 NZ_CP086619:67243-67461 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References