Detailed information of TA system TA182889

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 3425044..3425786
• Replicon: chromosome
• Accession: NZ_CP065534
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: C6C3X4
• Locus tag: I6N93_RS15135
• Protein ID: WP_015855199.1
• Coordinates: 3425307..3425786 (+)
• Length: 160 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A3N0G0N1
• Locus tag: I6N93_RS15130
• Protein ID: WP_019843650.1
• Coordinates: 3425044..3425316 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6N93_RS15100	3420077..3421129	+	1053	WP_085690248.1	site-specific tyrosine recombinase XerC	-
I6N93_RS15105	3421153..3421599	+	447	WP_197669173.1	hypothetical protein	-
I6N93_RS15110	3422061..3422162	+	102	Protein_2934	Rhs family protein	-
I6N93_RS15115	3422502..3422852	+	351	WP_085690284.1	hypothetical protein	-
I6N93_RS15120	3422922..3423260	-	339	WP_085690282.1	type I toxin-antitoxin system SymE family toxin	-
I6N93_RS15125	3423393..3424501	+	1109	WP_100059615.1	IS3 family transposase	-
I6N93_RS15130	3425044..3425316	+	273	WP_019843650.1	DUF1778 domain-containing protein	Antitoxin
I6N93_RS15135	3425307..3425786	+	480	WP_015855199.1	GNAT family N-acetyltransferase	Toxin
I6N93_RS15140	3426057..3426272	-	216	WP_051122262.1	helix-turn-helix domain-containing protein	-
I6N93_RS15145	3426368..3426634	+	267	WP_085685657.1	helix-turn-helix domain-containing protein	-
I6N93_RS15150	3426858..3428903	-	2046	WP_085685659.1	AAA family ATPase	-
I6N93_RS15155	3428896..3429432	-	537	WP_085685661.1	ash family protein	-
I6N93_RS15160	3429429..3429629	-	201	WP_026357784.1	AlpA family transcriptional regulator	-
I6N93_RS15165	3429632..3430513	-	882	WP_085685663.1	integrase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3423393..3423659	266
	-	inside	Prophage	-	vgrG	3398532..3434958	36426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (28-147)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-85)

Sequences

Toxin

Protein

• Length: 160 a.a.
• Molecular weight: 17538.30 Da
• Isoelectric Point: 9.5367

>T182889 WP_015855199.1 NZ_CP065534:3425307-3425786 [Lonsdalea populi]
VGITAPELLLPQHAVVDFHCSEPSLNEWLKRKALKNQTLGASRTFVVCEAGTQRVVGFYALASGSIQRQVAPGAFRRNMP
DPIPVLVLGRLAVDERYQRMGIGAGLLKDAVLRSRNVAQQVGNKALLVHALSDEAKAFYQYWGFVASEIQEHTLLLSLW

Nucleotide

• Length: 480 bp

>T182889 NZ_CP086618:c4614475-4614125 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGTGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10388.86 Da
• Isoelectric Point: 4.6702

>AT182889 WP_019843650.1 NZ_CP065534:3425044-3425316 [Lonsdalea populi]
MRTETKEMPINIRAKASQRELIDVAAKLLSKSRTEFILDAACREAEDVLLDQRLFLVNDEQYDAFIQVLESPVTDNPRVN
ALLNRKSPWE

Nucleotide

• Length: 273 bp

>AT182889 NZ_CP086618:c4614720-4614469 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A2K8QHE3

Antitoxin

• AlphaFold DB: A0A3N0G0N1