Detailed information of TA system TA182887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 2838721..2839311
• Replicon: chromosome
• Accession: NZ_CP065534
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: I6N93_RS12430
• Protein ID: WP_085690046.1
• Coordinates: 2838721..2839053 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: I6N93_RS12435
• Protein ID: WP_085690044.1
• Coordinates: 2839054..2839311 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6N93_RS12415	2834166..2835092	-	927	WP_085690050.1	ParA family protein	-
I6N93_RS12420	2835388..2836656	+	1269	WP_085690048.1	integrase arm-type DNA-binding domain-containing protein	-
I6N93_RS12425	2837079..2837948	-	870	WP_085690073.1	integrase domain-containing protein	-
I6N93_RS12430	2838721..2839053	-	333	WP_085690046.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
I6N93_RS12435	2839054..2839311	-	258	WP_085690044.1	antitoxin	Antitoxin
I6N93_RS12440	2839531..2840208	+	678	WP_197669156.1	ParA family protein	-
I6N93_RS12450	2841609..2841857	+	249	WP_139829925.1	hypothetical protein	-
I6N93_RS12455	2841902..2842045	+	144	WP_176222543.1	hypothetical protein	-
I6N93_RS12460	2842269..2843363	-	1095	WP_085687409.1	site-specific tyrosine recombinase XerC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2810710..2846860	36150
	-	flank	IS/Tn	-	-	2841117..2841383	266

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11768.54 Da
• Isoelectric Point: 10.9269

>T182887 WP_085690046.1 NZ_CP065534:c2839053-2838721 [Lonsdalea populi]
MDRGEIWLVSLDSTAGHEQSGKRPVLIVSPAPFNKITRLPVVVPVTSGGNFARTAGFAVSLDGTGTKTTGVVRCDQPRTI
DMAARNGRRLERIPDSIVNEVLARLGTILT

Nucleotide

• Length: 333 bp

>T182887 NZ_CP086618:c4122793-4122395 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.60 Da
• Isoelectric Point: 4.5368

>AT182887 WP_085690044.1 NZ_CP065534:c2839311-2839054 [Lonsdalea populi]
MYTTRLKKVGGSVMLAVPPAVLKTLDLSPDSEVGVTVDNGCLIIEPQKRPHYSLAELLAQCDPHAQVSDEDREWIDAPAT
GKEIL

Nucleotide

• Length: 258 bp

>AT182887 NZ_CP086618:c4123089-4122796 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA