Detailed information of TA system TA182884

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 912589..914309
• Replicon: chromosome
• Accession: NZ_CP065534
• Organism: Lonsdalea populi strain N-5-1

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: I6N93_RS04105
• Protein ID: WP_085686763.1
• Coordinates: 913653..914309 (-)
• Length: 219 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: I6N93_RS04100
• Protein ID: WP_085686765.1
• Coordinates: 912589..913656 (-)
• Length: 356 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6N93_RS04090	908757..909473	-	717	WP_085686769.1	M48 family metallopeptidase	-
I6N93_RS04095	909473..912568	-	3096	WP_085686767.1	HsdR family type I site-specific deoxyribonuclease	-
I6N93_RS04100	912589..913656	-	1068	WP_085686765.1	macro domain-containing protein	Antitoxin
I6N93_RS04105	913653..914309	-	657	WP_085686763.1	DUF4433 domain-containing protein	Toxin
I6N93_RS04110	914322..915665	-	1344	WP_099017421.1	restriction endonuclease subunit S	-
I6N93_RS04115	915683..918127	-	2445	WP_085686761.1	type I restriction-modification system subunit M	-
I6N93_RS04120	918168..918407	-	240	WP_085686759.1	transcription factor	-
I6N93_RS04125	918481..918753	+	273	WP_112111540.1	ash family protein	-
I6N93_RS04130	918808..919269	+	462	WP_085686757.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	864572..920911	56339

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (17-217)

Antitoxin

• Macro domain (18-129)

Sequences

Toxin

Protein

• Length: 219 a.a.
• Molecular weight: 24932.36 Da
• Isoelectric Point: 7.1574

>T182884 WP_085686763.1 NZ_CP065534:c914309-913653 [Lonsdalea populi]
MANNYSASLNPQKALIWRIVHRDNIPWILDNGLHCGNSPVQAGNWINIGNPELISKRAGHPVPVGTGGVLHDYVPFYFTP
FSPMLMNIHSGRGGIKQRPNEEIVILVSSLRTVAAQNVPFVFTDSHAYYNWANYYTELDNLNQIDWPILQARDFRRDPDD
PGKFERYQAEALIWQHCPIILLGGMVCYNDSVKLQLEQWLSQRNLTMPVHARAGWYFS

Nucleotide

• Length: 657 bp

>T182884 NZ_CP086618:3449053-3449439 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 356 a.a.
• Molecular weight: 40591.86 Da
• Isoelectric Point: 6.5864

>AT182884 WP_085686765.1 NZ_CP065534:c913656-912589 [Lonsdalea populi]
MITFTQGNLLDAPVEALVNTVNTVGVMGKGIALMFKERFPANMKAYALACKQKQVITGKMFITETGELMGPRWIVNFPTK
QHWRTDSRMEWIEDGLQDLRRFLIEKQVQSIAIPPLGAGNGGLNWPDVRAHIESALGDLQDVEILIYEPTEKYQNVAKST
GVKKLTPARAMIAELVRRYWVLGMECSLLEIQKLAWLLQRAIKLHQQENVLKLRFEAHNYGPYAHNLTHLLNALDGNYLK
AEKRIPDSQPLDVIWFNDREKDHVNTYLNSEAKTWLPALEQVSQLIDGFESPFGMELLATVDWLLTCDKCQPTLDSVKEG
LSQWPAGEHWASRKMKLFDDNNLQFAINRVMEFHC

Nucleotide

• Length: 1068 bp

>AT182884 NZ_CP086618:3449429-3449758 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA