Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-MazE
Location 2697142..2697775 Replicon chromosome
Accession NZ_CP065531
Organism Ralstonia solanacearum strain 362200

Toxin (Protein)


Gene name vapC Uniprot ID M4UIT8
Locus tag IMF25_RS12375 Protein ID WP_016725172.1
Coordinates 2697362..2697775 (+) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8XUX7
Locus tag IMF25_RS12370 Protein ID WP_011002949.1
Coordinates 2697142..2697375 (+) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMF25_RS12355 2692730..2694025 - 1296 WP_011002952.1 MFS transporter -
IMF25_RS12360 2694033..2695283 - 1251 WP_111375179.1 AGE family epimerase/isomerase -
IMF25_RS12365 2695478..2697085 + 1608 WP_020832850.1 glucan biosynthesis protein -
IMF25_RS12370 2697142..2697375 + 234 WP_011002949.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
IMF25_RS12375 2697362..2697775 + 414 WP_016725172.1 PIN domain-containing protein Toxin
IMF25_RS12380 2697750..2698376 - 627 WP_019718918.1 DUF1415 family protein -
IMF25_RS12385 2698567..2698938 + 372 WP_071015009.1 hypothetical protein -
IMF25_RS12390 2699000..2700496 - 1497 WP_011002945.1 glycerol kinase GlpK -
IMF25_RS12395 2700658..2702394 - 1737 WP_019718916.1 ABC transporter substrate-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-116)

Antitoxin

SpoVT-AbrB domain

(5-37)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 15087.42 Da        Isoelectric Point: 5.5348

>T182880 WP_016725172.1 NZ_CP065531:2697362-2697775 [Ralstonia solanacearum]
MPATEAFFDSNVVLYLLSADTAKADAAETLLMTGGVVSVQVLNETTHVMRRKLAMPWHAIETVQEAVRAQCRVEPLTLET
HDLGRRIAERYGLSVYDALIVAAALLAGCNVLYSEDMQHGLVIEQHLRIVNPFPARA

Download         Length: 414 bp

>T182880 NZ_CP065531:2697362-2697775 [Ralstonia solanacearum]
ATGCCCGCGACTGAGGCATTCTTCGACAGCAATGTCGTGCTGTACCTGCTGTCGGCCGACACCGCCAAGGCCGATGCGGC
GGAAACGCTGCTGATGACCGGCGGTGTCGTCAGCGTACAGGTACTGAACGAAACGACGCACGTGATGCGCCGCAAGCTGG
CCATGCCGTGGCACGCGATCGAAACGGTGCAGGAGGCCGTGCGTGCGCAATGCCGGGTCGAGCCGCTGACGCTCGAAACC
CATGATCTCGGACGGCGTATCGCAGAACGCTACGGATTGTCGGTCTATGACGCACTGATCGTCGCGGCGGCATTGCTTGC
CGGCTGCAACGTTCTGTATTCCGAGGATATGCAGCACGGCCTGGTGATCGAGCAGCATCTGCGCATCGTCAATCCTTTTC
CGGCACGGGCATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8790.98 Da        Isoelectric Point: 8.7287

>AT182880 WP_011002949.1 NZ_CP065531:2697142-2697375 [Ralstonia solanacearum]
MQVSKWGNSLAIRLPAAVVEALQLHEGDDVEVVIAGERSFGVRRKRAARELFDRIHQYRGKLPADFIFERDQANARD

Download         Length: 234 bp

>AT182880 NZ_CP065531:2697142-2697375 [Ralstonia solanacearum]
ATGCAAGTCTCGAAATGGGGCAACAGCCTCGCTATCCGGCTGCCGGCGGCCGTTGTGGAGGCGCTTCAGCTGCACGAAGG
CGACGACGTCGAGGTGGTGATCGCGGGCGAGCGCTCGTTTGGCGTCCGCCGCAAGCGCGCTGCGCGCGAGCTTTTCGACC
GGATCCACCAATATCGCGGCAAGCTGCCCGCCGATTTCATCTTCGAGCGCGACCAGGCCAATGCCCGCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB M4UIT8

Antitoxin

Source ID Structure
AlphaFold DB A0A223GR76

References