Detailed information of TA system TA182878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 622008..623594
• Replicon: chromosome
• Accession: NZ_CP065531
• Organism: Ralstonia solanacearum strain 362200

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A9E8CN90
• Locus tag: IMF25_RS02640
• Protein ID: WP_064047702.1
• Coordinates: 622257..623594 (+)
• Length: 446 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A223GLT8
• Locus tag: IMF25_RS02635
• Protein ID: WP_028853251.1
• Coordinates: 622008..622253 (+)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMF25_RS02610	618071..618997	-	927	WP_071013782.1	muramoyltetrapeptide carboxypeptidase	-
IMF25_RS02615	618994..619545	-	552	WP_016727233.1	tRNA adenosine(34) deaminase TadA	-
IMF25_RS02620	619565..620041	-	477	WP_011001399.1	6-carboxytetrahydropterin synthase QueD	-
IMF25_RS02625	620109..620747	-	639	WP_020831956.1	7-carboxy-7-deazaguanine synthase	-
IMF25_RS02630	621065..621904	+	840	WP_016725278.1	oxaloacetate decarboxylase	-
IMF25_RS02635	622008..622253	+	246	WP_028853251.1	helix-turn-helix transcriptional regulator	Antitoxin
IMF25_RS02640	622257..623594	+	1338	WP_064047702.1	type II toxin-antitoxin system HipA family toxin	Toxin
IMF25_RS02645	623591..624793	+	1203	WP_071013776.1	MFS transporter	-
IMF25_RS02650	625057..625785	+	729	WP_011001393.1	DsbA family protein	-
IMF25_RS02655	625798..626361	-	564	WP_080624831.1	RNA 2',3'-cyclic phosphodiesterase	-
IMF25_RS02660	626542..627240	-	699	WP_058908491.1	protocatechuate 3,4-dioxygenase subunit alpha	-
IMF25_RS02665	627237..627977	-	741	WP_011001390.1	protocatechuate 3,4-dioxygenase subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (5-107)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-63)

Sequences

Toxin

Protein

• Length: 446 a.a.
• Molecular weight: 48768.81 Da
• Isoelectric Point: 6.7232

>T182878 WP_064047702.1 NZ_CP065531:622257-623594 [Ralstonia solanacearum]
MAHPELHLWMNGEHVGIWTASRAGVPILRYDDAWLRSPNMRPLSLSLPIPAGVPELRGQVVDDYFDNLLPEAQPIRERVR
RRFGLASTDAAELLAAIGRDCVGAVQLLPPGMAPTGYDRIDSEPLTDSQVDTLLRNLTATPALGEDAGDPDAFRISIAGA
QEKTALLRIGDAWHRPLGATPTTHILKLPLGLIGNIRADMQDSVDNEWLCAQLLAAWGLEVARTEIARFGTSKVLAVERF
DRRWMPGNAWIARLPQEDFCQATGTPAHRKYENDGGPGMRACLDLLSASEHAIDDKRRFVLAQLAFWLLAATDGHAKNFS
LAIGRGGRFRMTPLYDVLSVWPIMGDGPNQLSPYRARLAMALRGKNAHARLADIHVRHWQALAAQSGVPDAFAGMQALVE
RVPAGLERVEAILPPDFSGRVWSSVRTGVLAQAERFLNELASVTP

Nucleotide

• Length: 1338 bp

>T182878 NZ_CP086618:2346683-2346786 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 8482.80 Da
• Isoelectric Point: 10.4693

>AT182878 WP_028853251.1 NZ_CP065531:622008-622253 [Ralstonia solanacearum]
MESLLSSAPQLSAHLKSLRKARGLTQAQLGALLGVSQNRIADIEKDPGAVGFEQLLRILHALGAQLTLRTSAPAAAAPAD
W

Nucleotide

• Length: 246 bp

>AT182878 NZ_CP086618:c2346824-2346553 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGCCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A223GLT8