Detailed information of TA system TA182877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 61255..61862
• Replicon: plasmid unnamed1
• Accession: NZ_CP065529
• Organism: Enterococcus faecium strain VRE3382

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: E3USU0
• Locus tag: ISU53_RS14435
• Protein ID: WP_002321032.1
• Coordinates: 61255..61605 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A829F3C0
• Locus tag: ISU53_RS14440
• Protein ID: WP_002287514.1
• Coordinates: 61599..61862 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ISU53_RS14410	56582..57883	+	1302	WP_197667084.1	PTS transporter subunit EIIC	-
ISU53_RS14415	58282..59472	+	1191	WP_002288791.1	IS256 family transposase	-
ISU53_RS14420	59641..60063	+	423	WP_002288787.1	hypothetical protein	-
ISU53_RS14425	60073..60276	+	204	WP_002299573.1	hypothetical protein	-
ISU53_RS14430	60617..61231	+	615	WP_002298870.1	recombinase family protein	-
ISU53_RS14435	61255..61605	-	351	WP_002321032.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
ISU53_RS14440	61599..61862	-	264	WP_002287514.1	hypothetical protein	Antitoxin
ISU53_RS14445	62203..62382	-	180	WP_002298867.1	hypothetical protein	-
ISU53_RS14450	62827..63264	-	438	WP_002287515.1	hypothetical protein	-
ISU53_RS14455	63280..63531	-	252	WP_002287517.1	hypothetical protein	-
ISU53_RS14460	63531..63737	-	207	WP_014748825.1	hypothetical protein	-
ISU53_RS14465	64180..64440	-	261	WP_014748826.1	hypothetical protein	-
ISU53_RS14470	64595..64717	+	123	WP_002295624.1	putative holin-like toxin	-
ISU53_RS14475	64876..65109	-	234	WP_077495153.1	hypothetical protein	-
ISU53_RS14480	65154..65639	-	486	WP_002287518.1	single-stranded DNA-binding protein	-
ISU53_RS14485	65642..66541	-	900	WP_002287519.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..215857	215857

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (6-111)

Antitoxin

• Antitoxin PemI-like (4-87)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 13255.28 Da
• Isoelectric Point: 8.0280

>T182877 WP_002321032.1 NZ_CP065529:c61605-61255 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Nucleotide

• Length: 351 bp

>T182877 NZ_CP086618:1303300-1303611 [Escherichia coli]
ATGCACATCATTTCGAAAGCGCCATTTGAAGAGTGTGCGCGCAAATATCCTAACGACGCTCTGGCTCTACACTCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCTAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10183.60 Da
• Isoelectric Point: 4.6865

>AT182877 WP_002287514.1 NZ_CP065529:c61862-61599 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Nucleotide

• Length: 264 bp

>AT182877 NZ_CP086618:1303608-1304027 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCAGCAATGCCGGTT
GGCGTGGCCTTACTTCGTACGCTGATTGATCAACATAATTTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
TCTGGTCAGTCAGATTTTGTCTGGGCAACGATCCCTGACGATATCCCATATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Toxin

• AlphaFold DB: A0A829F5H8

Antitoxin

• AlphaFold DB: A0A829F3C0