Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2343517..2344088 Replicon chromosome
Accession NZ_CP065527
Organism Enterococcus faecium strain VRE3382

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag ISU53_RS11520 Protein ID WP_002286801.1
Coordinates 2343517..2343858 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag ISU53_RS11525 Protein ID WP_002323011.1
Coordinates 2343858..2344088 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU53_RS11495 2338727..2340061 - 1335 WP_002286788.1 ABC transporter substrate-binding protein -
ISU53_RS11500 2340269..2341099 + 831 WP_002286789.1 manganese catalase family protein -
ISU53_RS11505 2341343..2341625 - 283 Protein_2211 transposase -
ISU53_RS11510 2341783..2341977 - 195 WP_002297028.1 hypothetical protein -
ISU53_RS11515 2342482..2342667 - 186 WP_002304207.1 hypothetical protein -
ISU53_RS11520 2343517..2343858 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISU53_RS11525 2343858..2344088 - 231 WP_002323011.1 hypothetical protein Antitoxin
ISU53_RS11530 2344375..2344713 + 339 WP_002286804.1 hypothetical protein -
ISU53_RS11535 2344918..2345157 - 240 WP_002321938.1 SOS response-associated peptidase family protein -
ISU53_RS11540 2345133..2345480 - 348 WP_002286808.1 SOS response-associated peptidase family protein -
ISU53_RS11545 2346212..2346793 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
ISU53_RS11550 2346976..2347602 - 627 WP_002286816.1 cysteine hydrolase -
ISU53_RS11555 2347624..2348955 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T182875 WP_002286801.1 NZ_CP065527:c2343858-2343517 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T182875 NZ_CP086618:1082812-1083219 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT182875 WP_002323011.1 NZ_CP065527:c2344088-2343858 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT182875 NZ_CP086618:1082565-1082831 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References