Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 21202..22339 Replicon plasmid unnamed3
Accession NZ_CP065526
Organism Enterococcus faecium strain VRE3389

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag ISU51_RS15835 Protein ID WP_001284311.1
Coordinates 21476..22339 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag ISU51_RS15830 Protein ID WP_000301765.1
Coordinates 21202..21474 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU51_RS15795 17022..17318 + 297 WP_000053907.1 replication control protein PrgN -
ISU51_RS15800 17342..18301 - 960 WP_002287225.1 IS30 family transposase -
ISU51_RS15805 18484..19335 + 852 WP_002287227.1 hypothetical protein -
ISU51_RS15810 19356..19639 + 284 Protein_24 hypothetical protein -
ISU51_RS15815 20001..20681 + 681 WP_002328852.1 IS6-like element IS1216 family transposase -
ISU51_RS15820 20735..20878 + 144 Protein_26 chromosome partitioning protein ParA -
ISU51_RS15825 20976..21185 + 210 WP_000527318.1 peptide-binding protein -
ISU51_RS15830 21202..21474 + 273 WP_000301765.1 antitoxin Antitoxin
ISU51_RS15835 21476..22339 + 864 WP_001284311.1 toxin zeta Toxin
ISU51_RS15840 22602..23339 - 738 WP_001038796.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
ISU51_RS15845 23464..23547 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
ISU51_RS15850 23596..23679 - 84 Protein_32 peptide-binding protein -
ISU51_RS15855 24089..24883 - 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
ISU51_RS15860 24976..25227 - 252 Protein_34 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid ant(6)-Ia / erm(B) / aph(3')-III - 1..25787 25787
- inside IScluster/Tn ant(6)-Ia / erm(B) / aph(3')-III - 186..24874 24688


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T182874 WP_001284311.1 NZ_CP065526:21476-22339 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T182874 NZ_CP086618:c957175-956801 [Escherichia coli]
ATGAAAACATTATCCGACACACATGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT182874 WP_000301765.1 NZ_CP065526:21202-21474 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT182874 NZ_CP086618:c957596-957222 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCAGATGCATACCATCCGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGTTACGTTTATCTGGCGGTTTATCCGACACCGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References