Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Txe-Axe/Txe-RelB
Location 107332..107851 Replicon plasmid unnamed1
Accession NZ_CP065525
Organism Enterococcus faecium strain VRE3389

Toxin (Protein)


Gene name Txe Uniprot ID Q848U6
Locus tag ISU51_RS15030 Protein ID WP_000588503.1
Coordinates 107332..107589 (-) Length 86 a.a.

Antitoxin (Protein)


Gene name Axe Uniprot ID Q83WJ8
Locus tag ISU51_RS15035 Protein ID WP_000388479.1
Coordinates 107582..107851 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU51_RS14995 102554..103234 + 681 WP_010861579.1 IS6-like element IS1216 family transposase -
ISU51_RS15000 103423..104007 - 585 WP_002299575.1 recombinase family protein -
ISU51_RS15005 104218..104421 - 204 WP_002299573.1 hypothetical protein -
ISU51_RS15010 104431..104853 - 423 WP_002288787.1 hypothetical protein -
ISU51_RS15015 105091..105705 + 615 WP_001261742.1 recombinase family protein -
ISU51_RS15020 105870..106694 - 825 WP_001809248.1 hypothetical protein -
ISU51_RS15025 106718..106873 - 156 WP_002347142.1 hypothetical protein -
ISU51_RS15030 107332..107589 - 258 WP_000588503.1 Txe/YoeB family addiction module toxin Toxin
ISU51_RS15035 107582..107851 - 270 WP_000388479.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
ISU51_RS15040 107982..108161 - 180 WP_001120991.1 hypothetical protein -
ISU51_RS15045 108542..109249 - 708 WP_002303117.1 hypothetical protein -
ISU51_RS15050 109242..109679 - 438 WP_002300851.1 hypothetical protein -
ISU51_RS15055 109694..109945 - 252 WP_002296238.1 hypothetical protein -
ISU51_RS15060 109945..110151 - 207 WP_002347218.1 hypothetical protein -
ISU51_RS15065 110588..110848 - 261 WP_002295623.1 hypothetical protein -
ISU51_RS15070 110923..111126 + 204 WP_002316074.1 putative holin-like toxin -
ISU51_RS15075 111267..111752 - 486 WP_002303313.1 single-stranded DNA-binding protein -
ISU51_RS15080 111755..112654 - 900 WP_002303312.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..227210 227210


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10206.53 Da        Isoelectric Point: 8.4369

>T182872 WP_000588503.1 NZ_CP065525:c107589-107332 [Enterococcus faecium]
MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLGKPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYS
AKDHY

Download         Length: 258 bp

>T182872 NZ_CP086618:c675551-675087 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10373.51 Da        Isoelectric Point: 4.4346

>AT182872 WP_000388479.1 NZ_CP065525:c107851-107582 [Enterococcus faecium]
MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKRDYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTH
DLIEVESDD

Download         Length: 270 bp

>AT182872 NZ_CP086618:c675886-675551 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXQ8

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZWU7

References