Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 36179..36786 Replicon plasmid unnamed2
Accession NZ_CP065524
Organism Enterococcus faecium strain VRE3389

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag ISU51_RS14115 Protein ID WP_002321032.1
Coordinates 36436..36786 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag ISU51_RS14110 Protein ID WP_002287514.1
Coordinates 36179..36442 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU51_RS14070 31214..32114 + 901 Protein_31 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
ISU51_RS14075 32117..32602 + 486 WP_002296241.1 single-stranded DNA-binding protein -
ISU51_RS14080 32968..33228 + 261 WP_002296240.1 hypothetical protein -
ISU51_RS14085 33669..33875 + 207 WP_002296239.1 hypothetical protein -
ISU51_RS14090 33875..34126 + 252 WP_002296238.1 hypothetical protein -
ISU51_RS14095 34141..34578 + 438 WP_002296237.1 hypothetical protein -
ISU51_RS14100 34571..35278 + 708 WP_002353391.1 hypothetical protein -
ISU51_RS14105 35659..35838 + 180 WP_002305761.1 hypothetical protein -
ISU51_RS14110 36179..36442 + 264 WP_002287514.1 hypothetical protein Antitoxin
ISU51_RS14115 36436..36786 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISU51_RS14120 36810..37424 - 615 WP_010729795.1 recombinase family protein -
ISU51_RS14125 38172..38375 + 204 WP_002299573.1 hypothetical protein -
ISU51_RS14130 38586..39170 + 585 WP_002299575.1 recombinase family protein -
ISU51_RS14135 39541..40866 + 1326 WP_002303115.1 Y-family DNA polymerase -
ISU51_RS14140 40859..41209 + 351 WP_002303114.1 hypothetical protein -
ISU51_RS14145 41206..41385 + 180 WP_001021554.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..94576 94576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T182871 WP_002321032.1 NZ_CP065524:36436-36786 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T182871 NZ_CP086618:263303-263410 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT182871 WP_002287514.1 NZ_CP065524:36179-36442 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT182871 NZ_CP086618:c263254-263191 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References