Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 2317324..2317895 Replicon chromosome
Accession NZ_CP065523
Organism Enterococcus faecium strain VRE3389

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag ISU51_RS11430 Protein ID WP_002286801.1
Coordinates 2317324..2317665 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag ISU51_RS11435 Protein ID WP_002323011.1
Coordinates 2317665..2317895 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ISU51_RS11405 2312535..2313869 - 1335 WP_002333419.1 ABC transporter substrate-binding protein -
ISU51_RS11410 2314076..2314906 + 831 WP_002286789.1 manganese catalase family protein -
ISU51_RS11415 2315150..2315432 - 283 Protein_2193 transposase -
ISU51_RS11420 2315590..2315784 - 195 WP_002295146.1 hypothetical protein -
ISU51_RS11425 2316289..2316474 - 186 WP_002304207.1 hypothetical protein -
ISU51_RS11430 2317324..2317665 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
ISU51_RS11435 2317665..2317895 - 231 WP_002323011.1 hypothetical protein Antitoxin
ISU51_RS11440 2318182..2318520 + 339 WP_002286804.1 hypothetical protein -
ISU51_RS11445 2318725..2319300 - 576 WP_002293673.1 SOS response-associated peptidase family protein -
ISU51_RS11450 2319909..2320490 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
ISU51_RS11455 2320673..2321299 - 627 WP_002333420.1 cysteine hydrolase -
ISU51_RS11460 2321321..2322652 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T182869 WP_002286801.1 NZ_CP065523:c2317665-2317324 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T182869 NZ_CP086618:262336-262443 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTCTGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT182869 WP_002323011.1 NZ_CP065523:c2317895-2317665 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT182869 NZ_CP086618:c262278-262224 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References