Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsdAT/-
Location 2217196..2217710 Replicon chromosome
Accession NZ_CP065515
Organism Staphylococcus aureus strain GDY8P96A

Toxin (Protein)


Gene name TsdT Uniprot ID A0A641A3V2
Locus tag IQE12_RS10970 Protein ID WP_000581793.1
Coordinates 2217196..2217405 (-) Length 70 a.a.

Antitoxin (Protein)


Gene name TsdA Uniprot ID W8UW13
Locus tag IQE12_RS10975 Protein ID WP_000138932.1
Coordinates 2217417..2217710 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQE12_RS10950 2212705..2214063 - 1359 WP_000611483.1 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase -
IQE12_RS10955 2214078..2215148 - 1071 WP_000159627.1 D-alanine--D-alanine ligase -
IQE12_RS10960 2215466..2216668 + 1203 WP_001109939.1 rod shape-determining protein RodA -
IQE12_RS10965 2216931..2217068 - 138 WP_000828354.1 hypothetical protein -
IQE12_RS10970 2217196..2217405 - 210 WP_000581793.1 heavy-metal-associated domain-containing protein Toxin
IQE12_RS10975 2217417..2217710 - 294 WP_000138932.1 copper-sensing transcriptional repressor CsoR Antitoxin
IQE12_RS10980 2217876..2219360 + 1485 WP_000571549.1 cardiolipin synthase -
IQE12_RS10985 2219388..2220035 + 648 WP_001187628.1 HD domain-containing protein -
IQE12_RS10990 2220649..2221521 - 873 WP_000725802.1 membrane protein insertase YidC -
IQE12_RS10995 2221607..2222248 - 642 WP_000483155.1 thiamine phosphate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Heavy metal-associated domain, HMA

(23-66)

Antitoxin

Metal-sensitive transcriptional repressor

(14-92)


Sequences

Toxin        


Download         Length: 70 a.a.        Molecular weight: 7809.75 Da        Isoelectric Point: 4.1008

>T182861 WP_000581793.1 NZ_CP065515:c2217405-2217196 [Staphylococcus aureus]
MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVTVSYETPANLNSIEKEIYDEGYKIVI

Download         Length: 210 bp

>T182861 NZ_CP086616:c4417466-4417179 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11197.82 Da        Isoelectric Point: 6.2243

>AT182861 WP_000138932.1 NZ_CP065515:c2217710-2217417 [Staphylococcus aureus]
MTEQDNAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDVLTQIRATRSALNSVAIKLLEQHMKSCIMNKVNQGAQ
EEAMEELLVTFQKLIKD

Download         Length: 294 bp

>AT182861 NZ_CP086616:c4417192-4416890 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A641A3V2

Antitoxin

Source ID Structure
AlphaFold DB W8UW13

References