Detailed information of TA system TA182860

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2197440..2197969
• Replicon: chromosome
• Accession: NZ_CP065515
• Organism: Staphylococcus aureus strain GDY8P96A

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: IQE12_RS10880
• Protein ID: WP_000621175.1
• Coordinates: 2197440..2197802 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: IQE12_RS10885
• Protein ID: WP_000948331.1
• Coordinates: 2197799..2197969 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQE12_RS10860	2194418..2195188	-	771	WP_001041108.1	RNA polymerase sigma factor SigB	-
IQE12_RS10865	2195163..2195642	-	480	WP_001190830.1	anti-sigma B factor RsbW	-
IQE12_RS10870	2195644..2195970	-	327	WP_001052491.1	anti-sigma factor antagonist	-
IQE12_RS10875	2196089..2197090	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
IQE12_RS10880	2197440..2197802	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
IQE12_RS10885	2197799..2197969	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
IQE12_RS10890	2198054..2199202	-	1149	WP_001281154.1	alanine racemase	-
IQE12_RS10895	2199268..2199627	-	360	WP_000581197.1	holo-ACP synthase	-
IQE12_RS10900	2199631..2200122	-	492	WP_001205912.1	PH domain-containing protein	-
IQE12_RS10905	2200109..2201692	-	1584	WP_063456354.1	PH domain-containing protein	-
IQE12_RS10910	2201685..2202164	-	480	WP_001287078.1	hypothetical protein	-
IQE12_RS10915	2202373..2202933	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T182860 WP_000621175.1 NZ_CP065515:c2197802-2197440 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T182860 NZ_CP086616:c4079597-4079199 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT182860 WP_000948331.1 NZ_CP065515:c2197969-2197799 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT182860 NZ_CP086616:c4079893-4079600 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAATCCAGCAAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CAGAACAAGAGGAGAAAAAACTGGTGAAGGCGCGCTTCCGTCCGAGTGCCGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1