Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 2844357..2844999 Replicon chromosome
Accession NZ_CP065509
Organism Bacillus sp. N447-1

Toxin (Protein)


Gene name pemK Uniprot ID J8CWW5
Locus tag IQ781_RS14260 Protein ID WP_000635963.1
Coordinates 2844357..2844707 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag IQ781_RS14265 Protein ID WP_000004570.1
Coordinates 2844712..2844999 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQ781_RS14245 (IQ781_14205) 2841293..2841751 - 459 WP_000344234.1 SprT family protein -
IQ781_RS14250 (IQ781_14210) 2841947..2842063 + 117 WP_001143642.1 cortex morphogenetic protein CmpA -
IQ781_RS14255 (IQ781_14215) 2842121..2844289 - 2169 WP_000426242.1 Tex family protein -
IQ781_RS14260 (IQ781_14220) 2844357..2844707 - 351 WP_000635963.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
IQ781_RS14265 (IQ781_14225) 2844712..2844999 - 288 WP_000004570.1 antitoxin EndoAI Antitoxin
IQ781_RS14270 (IQ781_14230) 2845308..2846477 - 1170 WP_000390615.1 alanine racemase -
IQ781_RS14275 (IQ781_14235) 2846595..2847545 - 951 WP_002004297.1 outer membrane lipoprotein carrier protein LolA -
IQ781_RS14280 (IQ781_14240) 2847702..2848061 - 360 WP_000583416.1 holo-ACP synthase -
IQ781_RS14285 (IQ781_14245) 2848155..2848727 + 573 WP_000908523.1 rhomboid family intramembrane serine protease -
IQ781_RS14290 (IQ781_14250) 2848720..2849682 - 963 WP_000961165.1 UV DNA damage repair endonuclease UvsE -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12974.12 Da        Isoelectric Point: 5.7168

>T182849 WP_000635963.1 NZ_CP065509:c2844707-2844357 [Bacillus sp. N447-1]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMIRVDEALQISLGLIDF

Download         Length: 351 bp

>T182849 NZ_CP086616:1245025-1245360 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAGCTCATTAAAGCCAAAATTAA
CGTGCTGATTGGGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT182849 WP_000004570.1 NZ_CP065509:c2844999-2844712 [Bacillus sp. N447-1]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT182849 NZ_CP086616:1244777-1245025 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4HKE
PDB 7BXY

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References