Detailed information of TA system TA182848

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 714979..715957
• Replicon: chromosome
• Accession: NZ_CP065509
• Organism: Bacillus sp. N447-1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: W8Y388
• Locus tag: IQ781_RS03500
• Protein ID: WP_000624977.1
• Coordinates: 715220..715957 (-)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: IQ781_RS03495
• Protein ID: WP_000237819.1
• Coordinates: 714979..715107 (-)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IQ781_RS03465 (IQ781_03445)	710468..710729	+	262	Protein_693	helix-turn-helix transcriptional regulator	-
IQ781_RS03470 (IQ781_03450)	710958..711560	-	603	WP_139846814.1	hypothetical protein	-
IQ781_RS03475 (IQ781_03455)	711586..712395	-	810	WP_139846815.1	papain-like cysteine protease family protein	-
IQ781_RS03480 (IQ781_03460)	712641..714110	-	1470	WP_000287556.1	beta-Ala-His dipeptidase	-
IQ781_RS03485 (IQ781_03465)	714321..714710	+	390	WP_000713863.1	YxeA family protein	-
IQ781_RS03490 (IQ781_03470)	714730..714906	-	177	WP_000852629.1	stage II sporulation protein SB	-
IQ781_RS03495 (IQ781_03475)	714979..715107	-	129	WP_000237819.1	hypothetical protein	Antitoxin
IQ781_RS03500 (IQ781_03480)	715220..715957	-	738	WP_000624977.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
IQ781_RS03505 (IQ781_03485)	716196..716933	-	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
IQ781_RS03510 (IQ781_03490)	717099..717581	-	483	WP_000191888.1	MarR family winged helix-turn-helix transcriptional regulator	-
IQ781_RS03515 (IQ781_03495)	717689..719374	+	1686	WP_074047527.1	alpha-keto acid decarboxylase family protein	-
IQ781_RS03520 (IQ781_03500)	719532..720227	-	696	WP_242808307.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28307.03 Da
• Isoelectric Point: 8.2998

>T182848 WP_000624977.1 NZ_CP065509:c715957-715220 [Bacillus sp. N447-1]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPAVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T182848 NZ_CP086616:1098284-1098691 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGCTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT182848 WP_000237819.1 NZ_CP065509:c715107-714979 [Bacillus sp. N447-1]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT182848 NZ_CP086616:1098037-1098303 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGATGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: W8Y388

Antitoxin

• AlphaFold DB: M1PKC2