Detailed information of TA system TA182846

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 4465393..4466012
• Replicon: chromosome
• Accession: NZ_CP065474
• Organism: Klebsiella michiganensis strain 7525

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: -
• Locus tag: I6F39_RS21395
• Protein ID: WP_197663535.1
• Coordinates: 4465794..4466012 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: J4WQ74
• Locus tag: I6F39_RS21390
• Protein ID: WP_009653563.1
• Coordinates: 4465393..4465767 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I6F39_RS21380	4460552..4461745	+	1194	WP_004136054.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
I6F39_RS21385	4461768..4464914	+	3147	WP_004848339.1	multidrug efflux RND transporter permease subunit	-
I6F39_RS21390	4465393..4465767	+	375	WP_009653563.1	Hha toxicity modulator TomB	Antitoxin
I6F39_RS21395	4465794..4466012	+	219	WP_197663535.1	hemolysin expression modulator Hha	Toxin
I6F39_RS21400	4466174..4466740	+	567	WP_004848331.1	maltose O-acetyltransferase	-
I6F39_RS21405	4466867..4467337	+	471	WP_004848329.1	YlaC family protein	-
I6F39_RS21410	4467312..4468766	-	1455	WP_049077911.1	PLP-dependent aminotransferase family protein	-
I6F39_RS21415	4468868..4469566	+	699	WP_128349260.1	GNAT family N-acetyltransferase	-
I6F39_RS21420	4469563..4469703	-	141	WP_003859006.1	type B 50S ribosomal protein L36	-
I6F39_RS21425	4469703..4469966	-	264	WP_004848323.1	type B 50S ribosomal protein L31	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8638.08 Da
• Isoelectric Point: 8.9008

>T182846 WP_197663535.1 NZ_CP065474:4465794-4466012 [Klebsiella michiganensis]
MSDKILTKVDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Nucleotide

• Length: 219 bp

>T182846 NZ_CP086616:c897148-896771 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14378.14 Da
• Isoelectric Point: 4.8989

>AT182846 WP_009653563.1 NZ_CP065474:4465393-4465767 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNASRENPVSLSC

Nucleotide

• Length: 375 bp

>AT182846 NZ_CP086616:c897606-897238 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J4R4I8