Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2079572..2080162 Replicon chromosome
Accession NZ_CP065474
Organism Klebsiella michiganensis strain 7525

Toxin (Protein)


Gene name pemK Uniprot ID J5V7J4
Locus tag I6F39_RS09965 Protein ID WP_009653321.1
Coordinates 2079830..2080162 (+) Length 111 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID J6I3K7
Locus tag I6F39_RS09960 Protein ID WP_004852307.1
Coordinates 2079572..2079829 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I6F39_RS09940 2075470..2076078 - 609 WP_197664187.1 glutathione S-transferase family protein -
I6F39_RS09945 2076255..2077181 + 927 WP_049078228.1 LysR family transcriptional regulator -
I6F39_RS09950 2077219..2078217 - 999 WP_049078227.1 aldo/keto reductase -
I6F39_RS09955 2078318..2079232 + 915 WP_086531142.1 LysR family transcriptional regulator -
I6F39_RS09960 2079572..2079829 + 258 WP_004852307.1 antitoxin Antitoxin
I6F39_RS09965 2079830..2080162 + 333 WP_009653321.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
I6F39_RS09975 2080485..2081942 + 1458 WP_032743138.1 EmmdR/YeeO family multidrug/toxin efflux MATE transporter -
I6F39_RS09985 2082905..2084359 - 1455 WP_197664188.1 AMP nucleosidase -
I6F39_RS09990 2084491..2084748 - 258 WP_009653336.1 histidine kinase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 2070507..2080162 9655


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-109)

Antitoxin

Antitoxin PemI-like

(4-84)


Sequences

Toxin        


Download         Length: 111 a.a.        Molecular weight: 11823.69 Da        Isoelectric Point: 10.5834

>T182837 WP_009653321.1 NZ_CP065474:2079830-2080162 [Klebsiella michiganensis]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPITSGGNFARTAGFTVSLEGAGIKTTGVIRCDQPRTI
DIAARNGKRLERIPDAVVNDVLARLDAIFS

Download         Length: 333 bp

>T182837 NZ_CP086615:2311421-2311723 [Spiribacter sp. E85]
GTGGCTGAAGTAATCTGGACGGAGCCCGCCGTCCAGGACCTCGATTCGATCGCCGAGTACATCGCACTCCACAATCCGCC
TGCCGCCAGACGCCTGATTGAGAACGTGTTCGAGAAGACCGGGCGGCTGGCGCGTTTCCCGCACTCGGGGCGCGTCCCGC
CCGAGCTGCCGGATTCGGTCTTCCGCGAAATGGTCGTGCCGCCCTGCCGCGTCTTCTACCGCACGGAGCAGGGCCGTGTG
CTCATTCTCCATGTGATGCGGGAGGAGCGACGGCTGCGTGCCTACCTGCTGGAAGGCGACTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9422.88 Da        Isoelectric Point: 4.4607

>AT182837 WP_004852307.1 NZ_CP065474:2079572-2079829 [Klebsiella michiganensis]
MYTTRLKKVGGSIMLAVPPAVLKTLELSTDSEVGMTINNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDREWIDAPAV
GKEIL

Download         Length: 258 bp

>AT182837 NZ_CP086615:2311176-2311433 [Spiribacter sp. E85]
ATGAAGGTCGAACTGGTGACGACCCTGAAGCGCCAGGCCACGAAGATCCTGGCCGAGCTGCATGAATCCAAGGAGCCCGT
GCTGATCACCGAGCACGGCAAGCCGTCTGCCTATCTCGTTGATGTGCAGGACTATGAGCTCATGCAGCGCAGGCTTGATC
TGCTGGAGGGGCTCTCCCGGGGAGAGCGGGCTGTCCTGGAAGGGCGAACGTACAGCCAGAACGAGGCCAGGGAAAGGCTC
GGTAAGTGGCTGAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB J5V7J4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3HDW8

References