Detailed information of TA system TA182831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 123905..124548
• Replicon: plasmid pPinheadLarry_03
• Accession: NZ_CP065470
• Organism: Klebsiella quasipneumoniae strain Pinhead Larry

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: I5M56_RS25845
• Protein ID: WP_021312475.1
• Coordinates: 124132..124548 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: A0A6B7Q5S4
• Locus tag: I5M56_RS25840
• Protein ID: WP_021312476.1
• Coordinates: 123905..124135 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
I5M56_RS25825	120004..121092	+	1089	WP_094716522.1	transcriptional regulator	-
I5M56_RS25830	121094..121963	+	870	WP_094716519.1	hypothetical protein	-
I5M56_RS25835	122020..123585	-	1566	WP_094716520.1	ATP-binding protein	-
I5M56_RS25840	123905..124135	+	231	WP_021312476.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
I5M56_RS25845	124132..124548	+	417	WP_021312475.1	type II toxin-antitoxin system VapC family toxin	Toxin
I5M56_RS25850	124583..124828	+	246	WP_065905238.1	hypothetical protein	-
I5M56_RS25855	124892..125110	+	219	WP_021312527.1	hypothetical protein	-
I5M56_RS25860	125189..125515	+	327	WP_124989605.1	hypothetical protein	-
I5M56_RS25865	125754..125969	+	216	WP_065808007.1	hypothetical protein	-
I5M56_RS25870	126010..126585	+	576	WP_065808008.1	hypothetical protein	-
I5M56_RS25875	126616..127110	+	495	WP_065808010.1	DNA-binding protein	-
I5M56_RS25880	127212..127499	-	288	WP_042938777.1	chorismate mutase	-
I5M56_RS25885	127654..128520	-	867	WP_052264179.1	hypothetical protein	-
I5M56_RS25890	128810..129076	+	267	Protein_153	ISNCY family transposase	-
I5M56_RS25895	129166..129261	+	96	Protein_154	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / aph(3')-Ia / sul2 / floR / aac(3)-IId / blaTEM-1B / aph(6)-Id / qnrB4 / blaDHA-1 / tet(A) / dfrA12 / aadA2 / qacE / sul1	-	1..148255	148255

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15033.44 Da
• Isoelectric Point: 7.8915

>T182831 WP_021312475.1 NZ_CP065470:124132-124548 [Klebsiella quasipneumoniae]
VKKTFMLDTNICSFIMREQPEAVLKRLEQAVLRGHRIVVSAITYSEMRFGATGPKASPRHIELVDAFCARLDAILPWDRA
AVDATTEIKVALRQAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFARVPGLVLEDWVN

Nucleotide

• Length: 417 bp

>T182831 NZ_CP086610:763987-764310 [Shinella zoogloeoides]
GTGAAGCGCGGCGATCTCGTCACCGTCGCAATCCCGGGGGATTTCGGAAAGCCGCGCCCGGCGCTGATCATCCAGTCGGA
CCTGTTTTCGGAGGCGGCGACGGTGACGGTCCTGCTCGTTTCCGGCACTTATCAGGATGCGCCGCTGCTGCGTATCCCCG
TCGAGCCGGACGCGCTGAATGGCCTCAAGAAATCGTCGCAGGTCATGGTCGATAAGGCCATGACGATCCGGCGCGAAAAG
ATCGGCGAGACCATCGGCCGGCTCGATGCAGAGACCATGGCGTCGGTTTCCCGCAGTCTCGCGCTTTTCCTGGGATTTGC
CTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8723.76 Da
• Isoelectric Point: 4.3410

>AT182831 WP_021312476.1 NZ_CP065470:123905-124135 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFDL

Nucleotide

• Length: 231 bp

>AT182831 NZ_CP086610:763769-763990 [Shinella zoogloeoides]
ATGCCGAAGGCTGTCAACGAAAGAGTGCAGAAGCGCCGGGACGCGTTGCGTGCGAACGGCTTGCGGCCGGTGCAGATGTG
GTTGCCCGATACGCGCCGCGGCGGCTTTGCGGAGGAATGCGCGCGGCAGGCGCGGATAATCGCCGCGGCCGAGGTGGACG
ACCGCGATCTCGACGGCCTGCTGGATGCCGCCTTCCTCGATCTTGTCGGCTCCGATACGTGA

Structures

Antitoxin

• AlphaFold DB: A0A6B7Q5S4