Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 81758..82497 Replicon plasmid pPinheadLarry_03
Accession NZ_CP065470
Organism Klebsiella quasipneumoniae strain Pinhead Larry

Toxin (Protein)


Gene name tacT Uniprot ID A0A5E1AVR5
Locus tag I5M56_RS25620 Protein ID WP_013087172.1
Coordinates 81758..82243 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag I5M56_RS25625 Protein ID WP_012540086.1
Coordinates 82231..82497 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
I5M56_RS25580 76944..77264 - 321 WP_004152720.1 type II toxin-antitoxin system RelE/ParE family toxin -
I5M56_RS25585 77345..77569 - 225 WP_004152719.1 hypothetical protein -
I5M56_RS25590 77580..77792 - 213 WP_004152718.1 hypothetical protein -
I5M56_RS25595 77853..78209 - 357 WP_004152717.1 hypothetical protein -
I5M56_RS25600 78845..79195 - 351 WP_023320095.1 hypothetical protein -
I5M56_RS25605 79192..79464 - 273 WP_004182067.1 hypothetical protein -
I5M56_RS25610 80158..80319 - 162 WP_004118124.1 type I toxin-antitoxin system Hok family toxin -
I5M56_RS25615 80389..81468 + 1080 WP_009653230.1 IS481 family transposase -
I5M56_RS25620 81758..82243 - 486 WP_013087172.1 GNAT family N-acetyltransferase Toxin
I5M56_RS25625 82231..82497 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
I5M56_RS25630 82796..83119 + 324 WP_012540250.1 metalloregulator ArsR/SmtB family transcription factor -
I5M56_RS25635 83121..83834 + 714 WP_012540184.1 arsenical resistance protein ArsH -
I5M56_RS25640 83843..84388 + 546 WP_012540227.1 sigma-70 family RNA polymerase sigma factor -
I5M56_RS25645 84464..84826 + 363 WP_094716534.1 arsenic metallochaperone ArsD family protein -
I5M56_RS25650 84853..86604 + 1752 WP_162866166.1 arsenical pump-driving ATPase -
I5M56_RS25655 86729..87265 + 537 WP_012540238.1 GNAT family N-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(A) / aph(3')-Ia / sul2 / floR / aac(3)-IId / blaTEM-1B / aph(6)-Id / qnrB4 / blaDHA-1 / tet(A) / dfrA12 / aadA2 / qacE / sul1 - 1..148255 148255
- flank IS/Tn - - 80389..81468 1079


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-152)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 162 a.a.        Molecular weight: 17810.62 Da        Isoelectric Point: 9.8560

>T182830 WP_013087172.1 NZ_CP065470:c82243-81758 [Klebsiella quasipneumoniae]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQQRTLFLKLP
Q

Download         Length: 486 bp

>T182830 NZ_CP086609:c20594-20289 [Escherichia fergusonii]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGTCGTTATCGTCTGTTTGTGGATGTACAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGATAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGTGATGAAAGCTGGCGCATGATGACCTCCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGGGAAAATGACATCAAAAACGCCATTAACCTGATGTTTTGGGGAATATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT182830 WP_012540086.1 NZ_CP065470:c82497-82231 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT182830 NZ_CP086609:c20814-20596 [Escherichia fergusonii]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5E1AVR5

Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References